Transcriptomic and epigenetic analysis of breast cancer stem cells

Guochao Li; Dong Wang; Wencui Ma; Ke An; Zongzhi Liu; Xinyu Wang; Caiyun Yang; Fengxia Du; Xiao Han; Shuang Chang; Hui Yu; Zilong Zhang; Zitong Zhao; Yan Zhang; Junyun Wang; Yingli Sun

doi:10.2217/epi-2018-0008

Transcriptomic and epigenetic analysis of breast cancer stem cells

Guochao Li
, Dong Wang
, Wencui Ma
, Ke An
, Zongzhi Liu
, Xinyu Wang
, Caiyun Yang
, Fengxia Du
, Xiao Han
, Shuang Chang
, Hui Yu
, Zilong Zhang
, Zitong Zhao
, Yan Zhang
, Junyun Wang^*
, Yingli Sun

^*Corresponding author for this work

CAS - Beijing Institute of Genomics
University of Chinese Academy of Sciences
Heze Third People's Hospital
Harbin Medical University

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Aim: Cancer stem cells (CSCs) drive triple-negative breast cancer recurrence via their properties of self-renewal, invasiveness and radio/chemotherapy resistance. This study examined how CSCs might sustain these properties. Materials & methods: Transcriptomes, DNA methylomes and histone modifications were compared between CSCs and non CSCs. Results: Transcriptome analysis revealed several pathways that were activated in CSCs, whereas cell cycle regulation pathways were inhibited. Cell development and signaling genes were differentially methylated, with histone methylation analysis suggesting distinct H3K4me2 and H3K27me3 enrichment profiles. An integrated analysis revealed several tumor suppressor genes downregulated in CSCs. Conclusion: Differential activation of various signaling pathways and genes contributes to the tumor-promoting properties of CSCs. Therapeutic targets identified in the analysis may contribute to improving treatment options for patients.

Original language	English
Pages (from-to)	765-783
Number of pages	19
Journal	Epigenomics
Volume	10
Issue number	6
DOIs	https://doi.org/10.2217/epi-2018-0008
State	Published - Jun 2018
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

DNA methylation
H3K27me3
H3K4me2
breast cancer stem cells
epigenome
histone modification
multiple omics analysis
transcriptome

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10.2217/epi-2018-0008

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@article{58a3c95a1d814af7836ad24e9d55d9e2,

title = "Transcriptomic and epigenetic analysis of breast cancer stem cells",

abstract = "Aim: Cancer stem cells (CSCs) drive triple-negative breast cancer recurrence via their properties of self-renewal, invasiveness and radio/chemotherapy resistance. This study examined how CSCs might sustain these properties. Materials \& methods: Transcriptomes, DNA methylomes and histone modifications were compared between CSCs and non CSCs. Results: Transcriptome analysis revealed several pathways that were activated in CSCs, whereas cell cycle regulation pathways were inhibited. Cell development and signaling genes were differentially methylated, with histone methylation analysis suggesting distinct H3K4me2 and H3K27me3 enrichment profiles. An integrated analysis revealed several tumor suppressor genes downregulated in CSCs. Conclusion: Differential activation of various signaling pathways and genes contributes to the tumor-promoting properties of CSCs. Therapeutic targets identified in the analysis may contribute to improving treatment options for patients.",

keywords = "DNA methylation, H3K27me3, H3K4me2, breast cancer stem cells, epigenome, histone modification, multiple omics analysis, transcriptome",

author = "Guochao Li and Dong Wang and Wencui Ma and Ke An and Zongzhi Liu and Xinyu Wang and Caiyun Yang and Fengxia Du and Xiao Han and Shuang Chang and Hui Yu and Zilong Zhang and Zitong Zhao and Yan Zhang and Junyun Wang and Yingli Sun",

note = "Publisher Copyright: {\textcopyright} 2018 Future Medicine Ltd.",

year = "2018",

month = jun,

doi = "10.2217/epi-2018-0008",

language = "英语",

volume = "10",

pages = "765--783",

journal = "Epigenomics",

issn = "1750-1911",

publisher = "Taylor and Francis Ltd.",

number = "6",

}

TY - JOUR

T1 - Transcriptomic and epigenetic analysis of breast cancer stem cells

AU - Li, Guochao

AU - Wang, Dong

AU - Ma, Wencui

AU - An, Ke

AU - Liu, Zongzhi

AU - Wang, Xinyu

AU - Yang, Caiyun

AU - Du, Fengxia

AU - Han, Xiao

AU - Chang, Shuang

AU - Yu, Hui

AU - Zhang, Zilong

AU - Zhao, Zitong

AU - Zhang, Yan

AU - Wang, Junyun

AU - Sun, Yingli

PY - 2018/6

Y1 - 2018/6

N2 - Aim: Cancer stem cells (CSCs) drive triple-negative breast cancer recurrence via their properties of self-renewal, invasiveness and radio/chemotherapy resistance. This study examined how CSCs might sustain these properties. Materials & methods: Transcriptomes, DNA methylomes and histone modifications were compared between CSCs and non CSCs. Results: Transcriptome analysis revealed several pathways that were activated in CSCs, whereas cell cycle regulation pathways were inhibited. Cell development and signaling genes were differentially methylated, with histone methylation analysis suggesting distinct H3K4me2 and H3K27me3 enrichment profiles. An integrated analysis revealed several tumor suppressor genes downregulated in CSCs. Conclusion: Differential activation of various signaling pathways and genes contributes to the tumor-promoting properties of CSCs. Therapeutic targets identified in the analysis may contribute to improving treatment options for patients.

AB - Aim: Cancer stem cells (CSCs) drive triple-negative breast cancer recurrence via their properties of self-renewal, invasiveness and radio/chemotherapy resistance. This study examined how CSCs might sustain these properties. Materials & methods: Transcriptomes, DNA methylomes and histone modifications were compared between CSCs and non CSCs. Results: Transcriptome analysis revealed several pathways that were activated in CSCs, whereas cell cycle regulation pathways were inhibited. Cell development and signaling genes were differentially methylated, with histone methylation analysis suggesting distinct H3K4me2 and H3K27me3 enrichment profiles. An integrated analysis revealed several tumor suppressor genes downregulated in CSCs. Conclusion: Differential activation of various signaling pathways and genes contributes to the tumor-promoting properties of CSCs. Therapeutic targets identified in the analysis may contribute to improving treatment options for patients.

KW - DNA methylation

KW - H3K27me3

KW - H3K4me2

KW - breast cancer stem cells

KW - epigenome

KW - histone modification

KW - multiple omics analysis

KW - transcriptome

UR - https://www.scopus.com/pages/publications/85049658355

U2 - 10.2217/epi-2018-0008

DO - 10.2217/epi-2018-0008

M3 - 文章

C2 - 29480027

AN - SCOPUS:85049658355

SN - 1750-1911

VL - 10

SP - 765

EP - 783

JO - Epigenomics

JF - Epigenomics

IS - 6

ER -

Transcriptomic and epigenetic analysis of breast cancer stem cells

Abstract

UN SDGs

Keywords

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