Trajectory Tracking of Zebrafish Larvae Group

Alongside with the detection and the location of zebrafish larva’s key organs, another practical but more challenging task in the research of micromanipulation of zebrafish larva is the tracking of its movement, which is the prerequisite of the realization of its immobilization. In spite of the fact that quantities of algorithms about object tracking have already existed and new research results are proposed every year, the tracking of fast-moving zebrafish larva is still an open question due to its deformable body and extremely high speed. At the same time, for zebrafish larvae that have been micromanipulated, tracking tests are needed to study their behavioral patterns and physiological changes. Usually, this part is manually marked frame-by-frame for the zebrafish of the video sequence, which is inefficient and prone to cause tracking errors due to the fatigue of the experimenter. At present, the existing tracking systems on the market are aimed at macroscopic organisms such as adult fish and mice. The special problems in the tracking of larva fish have not been well solved, and a set of accurate tracking algorithms for larva are urgently needed. In this chapter, our latest research results on zebrafish larva tracking will be introduced.