TPF imaging of Rhodamine B at different detection windows

Broadband two-photon fluorescence (TPF) imaging system based on femtosecond oscillator is built. Broadband TPF signal generated from Rhodamine B molecule is detected at different detection windows. Both TPF signals detected at short wavelength detection window of 545-615 nm and long wavelength detection window of 642-708 nm are used for TPF imaging. Through switching detection window from short wavelength to long wavelength, deepened imaging depth is achieved. The TPF imaging results, with single 900 × 900 pixels frame taking time of 4-8 s, demonstrate high performance and the good reliability of the imaging system.