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Three-dimensional imaging and tracking for constrained Brownian motion of single nano-scaled particles in solution

  • Danying Lin*
  • , Wanyun Ma
  • , Xiaochen Liu
  • , Yunxu Sun
  • , Yao Wu
  • *Corresponding author for this work
  • Tsinghua University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Objective-type total internal reflection fluorescence technique and Gaussian fit single particle tracking algorithm have been used to study the constrained Brownian motion characteristics of single fluorescent microspheres of 40 nm diameter in real-time. Threedimensional tracking has been realized from the twodimensional images by utilizing the exponential decay of the fluorescence intensity excited by the evanescent field. Trajectory analyses have shown that single fluorescent beads diffused in a constrained Brownian mode with a diffusion coefficient a little smaller than theoretical estimates in the plane-parallel direction.

Original languageEnglish
Title of host publicationProceedings - 1st International Congress on Image and Signal Processing, CISP 2008
Pages244-248
Number of pages5
DOIs
StatePublished - 2008
Externally publishedYes
Event1st International Congress on Image and Signal Processing, CISP 2008 - Sanya, Hainan, China
Duration: 27 May 200830 May 2008

Publication series

NameProceedings - 1st International Congress on Image and Signal Processing, CISP 2008
Volume4

Conference

Conference1st International Congress on Image and Signal Processing, CISP 2008
Country/TerritoryChina
CitySanya, Hainan
Period27/05/0830/05/08

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