The role of microstructure of extracellular proteins in dewaterability of alkaline pretreatment sludge during bioleaching

Alkaline pre-treatment is known to enhance the acid production efficiency of sludge but adversely affects its dewatering performance. In this study, the improvement of sludge dewaterability by a novel bioleaching system with inoculating domesticated acidified sludge (AS) and its underlying mechanism were investigated. The results showed that although the addition of Fe²⁺ and the reduction of pH improved the dewatering performance of sludge, their effects were inferior to that of AS + Fe. The addition of AS and Fe²⁺ significantly reduced the specific resistance to filtration and capillary suction time of the sludge by 98.6 % and 95.5 %, respectively. This improvement in dewatering performance was achieved through the combined actions of bio-acidification, bio-oxidation, and bio-flocculation. Remarkably, under alkaline pH, microorganisms in AS remained active, leading to the formation of iron-based bioflocculants, along with a rapid pH decrease. These bioflocculants, in combination with protein (PN) in tightly bound extracellular polymeric substances (TB-EPS) through amide bonding, transformed TB-EPS from extractable to non-extractable form, reducing PN content from 12.1 mg g⁻¹DS to 5.09 mg g⁻¹DS and altering the protein's secondary structure. Consequently, the gel-like TB-EPS matrix effectively broke down, releasing cellular water and significantly enhancing sludge dewaterability.