The Effect of Compatible Solutes on the Cell Membrane of Lactobacillus delbrueckii subsp. bulgaricus 3

J. L. Sun; X. Han; Y. Wang; F. X. Lin; X. Chen

doi:10.1134/S0026261722601713

The Effect of Compatible Solutes on the Cell Membrane of Lactobacillus delbrueckii subsp. bulgaricus 3

J. L. Sun
, X. Han^*
, Y. Wang
, F. X. Lin
, X. Chen

^*Corresponding author for this work

School of Chemistry and Chemical Engineering, Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Abstract: Compatible solutes can reduce the impact of salt stress on bacteria. This work describes the changes in Lactobacillus delbrueckii subsp. bulgaricus 3 (L. bulgaricus 3) cell morphology, viability, cell membrane permeability, Zeta potential, and fluidity under salt stress. The results showed that compatible solutes shortened the transition of bacterial cells to the stationary phase under salt stress from 25 to 22 h. The cell diameter decreased from 831 to 515 nm. Compatible solutes increased the number of bacteria and promoted cell growth. The number of viable bacteria increased from 4.33 × 10⁶ to 5.71 × 10⁸ CFU/mL. Application of compatible solutes also improved stability of the cell membrane and maintained integrity under salt stress. According to the results of fluorescence anisotropy analysis, compatible solutes increased the fluidity of the cell membrane under salt stress. These observations suggested that compatible solutes could decrease cell growth inhibition under salt stress by changing cell morphology and the cell membrane properties.

Original language	English
Pages (from-to)	379-388
Number of pages	10
Journal	Microbiology (Russian Federation)
Volume	92
Issue number	3
DOIs	https://doi.org/10.1134/S0026261722601713
State	Published - Jun 2023
Externally published	Yes

Keywords

L. bulgaricus
cell membrane
compatible solutes
salt stress
viable count

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10.1134/S0026261722601713

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title = "The Effect of Compatible Solutes on the Cell Membrane of Lactobacillus delbrueckii subsp. bulgaricus 3",

abstract = "Abstract: Compatible solutes can reduce the impact of salt stress on bacteria. This work describes the changes in Lactobacillus delbrueckii subsp. bulgaricus 3 (L. bulgaricus 3) cell morphology, viability, cell membrane permeability, Zeta potential, and fluidity under salt stress. The results showed that compatible solutes shortened the transition of bacterial cells to the stationary phase under salt stress from 25 to 22 h. The cell diameter decreased from 831 to 515 nm. Compatible solutes increased the number of bacteria and promoted cell growth. The number of viable bacteria increased from 4.33 × 106 to 5.71 × 108 CFU/mL. Application of compatible solutes also improved stability of the cell membrane and maintained integrity under salt stress. According to the results of fluorescence anisotropy analysis, compatible solutes increased the fluidity of the cell membrane under salt stress. These observations suggested that compatible solutes could decrease cell growth inhibition under salt stress by changing cell morphology and the cell membrane properties.",

keywords = "L. bulgaricus, cell membrane, compatible solutes, salt stress, viable count",

author = "Sun, \{J. L.\} and X. Han and Y. Wang and Lin, \{F. X.\} and X. Chen",

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year = "2023",

month = jun,

doi = "10.1134/S0026261722601713",

language = "英语",

volume = "92",

pages = "379--388",

journal = "Microbiology (Russian Federation)",

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T1 - The Effect of Compatible Solutes on the Cell Membrane of Lactobacillus delbrueckii subsp. bulgaricus 3

AU - Sun, J. L.

AU - Han, X.

AU - Wang, Y.

AU - Lin, F. X.

AU - Chen, X.

PY - 2023/6

Y1 - 2023/6

N2 - Abstract: Compatible solutes can reduce the impact of salt stress on bacteria. This work describes the changes in Lactobacillus delbrueckii subsp. bulgaricus 3 (L. bulgaricus 3) cell morphology, viability, cell membrane permeability, Zeta potential, and fluidity under salt stress. The results showed that compatible solutes shortened the transition of bacterial cells to the stationary phase under salt stress from 25 to 22 h. The cell diameter decreased from 831 to 515 nm. Compatible solutes increased the number of bacteria and promoted cell growth. The number of viable bacteria increased from 4.33 × 106 to 5.71 × 108 CFU/mL. Application of compatible solutes also improved stability of the cell membrane and maintained integrity under salt stress. According to the results of fluorescence anisotropy analysis, compatible solutes increased the fluidity of the cell membrane under salt stress. These observations suggested that compatible solutes could decrease cell growth inhibition under salt stress by changing cell morphology and the cell membrane properties.

AB - Abstract: Compatible solutes can reduce the impact of salt stress on bacteria. This work describes the changes in Lactobacillus delbrueckii subsp. bulgaricus 3 (L. bulgaricus 3) cell morphology, viability, cell membrane permeability, Zeta potential, and fluidity under salt stress. The results showed that compatible solutes shortened the transition of bacterial cells to the stationary phase under salt stress from 25 to 22 h. The cell diameter decreased from 831 to 515 nm. Compatible solutes increased the number of bacteria and promoted cell growth. The number of viable bacteria increased from 4.33 × 106 to 5.71 × 108 CFU/mL. Application of compatible solutes also improved stability of the cell membrane and maintained integrity under salt stress. According to the results of fluorescence anisotropy analysis, compatible solutes increased the fluidity of the cell membrane under salt stress. These observations suggested that compatible solutes could decrease cell growth inhibition under salt stress by changing cell morphology and the cell membrane properties.

KW - L. bulgaricus

KW - cell membrane

KW - compatible solutes

KW - salt stress

KW - viable count

UR - https://www.scopus.com/pages/publications/85161028059

U2 - 10.1134/S0026261722601713

DO - 10.1134/S0026261722601713

M3 - 文章

AN - SCOPUS:85161028059

SN - 0026-2617

VL - 92

SP - 379

EP - 388

JO - Microbiology (Russian Federation)

JF - Microbiology (Russian Federation)

IS - 3

ER -

The Effect of Compatible Solutes on the Cell Membrane of Lactobacillus delbrueckii subsp. bulgaricus 3

Abstract

Keywords

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