The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system

Yunhua Xiao; Yong Dong Xu; Weiling Dong; Yili Liang; Fenliang Fan; Xiaoxia Zhang; Xian Zhang; Jiaojiao Niu; Liyuan Ma; Siyuan She; Zhili He; Xueduan Liu; Huaqun Yin

doi:10.1007/s00253-015-6881-x

The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system

Yunhua Xiao
, Yong Dong Xu
, Weiling Dong
, Yili Liang
, Fenliang Fan
, Xiaoxia Zhang
, Xian Zhang
, Jiaojiao Niu
, Liyuan Ma
, Siyuan She
, Zhili He
, Xueduan Liu
, Huaqun Yin^*

^*Corresponding author for this work

School of Minerals Processing and Bioengineering
Central South University
School of Computer Science and Technology, Harbin Institute of Technology
Key Laboratory of Plant Nutrition and Fertilizer
Chinese Academy of Agricultural Sciences
Ministry of Agriculture of the People's Republic of China
University of Oklahoma

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

This study used an artificial enrichment microbial consortium to examine the effects of different substrate conditions on microbial diversity, composition, and function (e.g., zinc leaching efficiency) through adding pyrite (SP group), chalcopyrite (SC group), or both (SPC group) in sphalerite bioleaching systems. 16S rRNA gene sequencing analysis showed that microbial community structures and compositions dramatically changed with additions of pyrite or chalcopyrite during the sphalerite bioleaching process. Shannon diversity index showed a significantly increase in the SP (1.460), SC (1.476), and SPC (1.341) groups compared with control (sphalerite group, 0.624) on day 30, meanwhile, zinc leaching efficiencies were enhanced by about 13.4, 2.9, and 13.2 %, respectively. Also, additions of pyrite or chalcopyrite could increase electric potential (ORP) and the concentrations of Fe³⁺ and H⁺, which were the main factors shaping microbial community structures by Mantel test analysis. Linear regression analysis showed that ORP, Fe³⁺ concentration, and pH were significantly correlated to zinc leaching efficiency and microbial diversity. In addition, we found that leaching efficiency showed a positive and significant relationship with microbial diversity. In conclusion, our results showed that the complicated substrates could significantly enhance microbial diversity and activity of function.

Original language	English
Pages (from-to)	10311-10322
Number of pages	12
Journal	Applied Microbiology and Biotechnology
Volume	99
Issue number	23
DOIs	https://doi.org/10.1007/s00253-015-6881-x
State	Published - 1 Dec 2015
Externally published	Yes

Keywords

16S rRNA gene sequencing
Artificial enrichment microbial consortium
Linear regression analysis
Shannon diversity index
Sphalerite bioleaching

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10.1007/s00253-015-6881-x

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title = "The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system",

abstract = "This study used an artificial enrichment microbial consortium to examine the effects of different substrate conditions on microbial diversity, composition, and function (e.g., zinc leaching efficiency) through adding pyrite (SP group), chalcopyrite (SC group), or both (SPC group) in sphalerite bioleaching systems. 16S rRNA gene sequencing analysis showed that microbial community structures and compositions dramatically changed with additions of pyrite or chalcopyrite during the sphalerite bioleaching process. Shannon diversity index showed a significantly increase in the SP (1.460), SC (1.476), and SPC (1.341) groups compared with control (sphalerite group, 0.624) on day 30, meanwhile, zinc leaching efficiencies were enhanced by about 13.4, 2.9, and 13.2 \%, respectively. Also, additions of pyrite or chalcopyrite could increase electric potential (ORP) and the concentrations of Fe3+ and H+, which were the main factors shaping microbial community structures by Mantel test analysis. Linear regression analysis showed that ORP, Fe3+ concentration, and pH were significantly correlated to zinc leaching efficiency and microbial diversity. In addition, we found that leaching efficiency showed a positive and significant relationship with microbial diversity. In conclusion, our results showed that the complicated substrates could significantly enhance microbial diversity and activity of function.",

keywords = "16S rRNA gene sequencing, Artificial enrichment microbial consortium, Linear regression analysis, Shannon diversity index, Sphalerite bioleaching",

author = "Yunhua Xiao and Xu, \{Yong Dong\} and Weiling Dong and Yili Liang and Fenliang Fan and Xiaoxia Zhang and Xian Zhang and Jiaojiao Niu and Liyuan Ma and Siyuan She and Zhili He and Xueduan Liu and Huaqun Yin",

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T1 - The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system

AU - Xiao, Yunhua

AU - Xu, Yong Dong

AU - Dong, Weiling

AU - Liang, Yili

AU - Fan, Fenliang

AU - Zhang, Xiaoxia

AU - Zhang, Xian

AU - Niu, Jiaojiao

AU - Ma, Liyuan

AU - She, Siyuan

AU - He, Zhili

AU - Liu, Xueduan

AU - Yin, Huaqun

PY - 2015/12/1

Y1 - 2015/12/1

N2 - This study used an artificial enrichment microbial consortium to examine the effects of different substrate conditions on microbial diversity, composition, and function (e.g., zinc leaching efficiency) through adding pyrite (SP group), chalcopyrite (SC group), or both (SPC group) in sphalerite bioleaching systems. 16S rRNA gene sequencing analysis showed that microbial community structures and compositions dramatically changed with additions of pyrite or chalcopyrite during the sphalerite bioleaching process. Shannon diversity index showed a significantly increase in the SP (1.460), SC (1.476), and SPC (1.341) groups compared with control (sphalerite group, 0.624) on day 30, meanwhile, zinc leaching efficiencies were enhanced by about 13.4, 2.9, and 13.2 %, respectively. Also, additions of pyrite or chalcopyrite could increase electric potential (ORP) and the concentrations of Fe3+ and H+, which were the main factors shaping microbial community structures by Mantel test analysis. Linear regression analysis showed that ORP, Fe3+ concentration, and pH were significantly correlated to zinc leaching efficiency and microbial diversity. In addition, we found that leaching efficiency showed a positive and significant relationship with microbial diversity. In conclusion, our results showed that the complicated substrates could significantly enhance microbial diversity and activity of function.

AB - This study used an artificial enrichment microbial consortium to examine the effects of different substrate conditions on microbial diversity, composition, and function (e.g., zinc leaching efficiency) through adding pyrite (SP group), chalcopyrite (SC group), or both (SPC group) in sphalerite bioleaching systems. 16S rRNA gene sequencing analysis showed that microbial community structures and compositions dramatically changed with additions of pyrite or chalcopyrite during the sphalerite bioleaching process. Shannon diversity index showed a significantly increase in the SP (1.460), SC (1.476), and SPC (1.341) groups compared with control (sphalerite group, 0.624) on day 30, meanwhile, zinc leaching efficiencies were enhanced by about 13.4, 2.9, and 13.2 %, respectively. Also, additions of pyrite or chalcopyrite could increase electric potential (ORP) and the concentrations of Fe3+ and H+, which were the main factors shaping microbial community structures by Mantel test analysis. Linear regression analysis showed that ORP, Fe3+ concentration, and pH were significantly correlated to zinc leaching efficiency and microbial diversity. In addition, we found that leaching efficiency showed a positive and significant relationship with microbial diversity. In conclusion, our results showed that the complicated substrates could significantly enhance microbial diversity and activity of function.

KW - 16S rRNA gene sequencing

KW - Artificial enrichment microbial consortium

KW - Linear regression analysis

KW - Shannon diversity index

KW - Sphalerite bioleaching

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DO - 10.1007/s00253-015-6881-x

M3 - 文章

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AN - SCOPUS:84947027099

SN - 0175-7598

VL - 99

SP - 10311

EP - 10322

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 23

ER -

The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system

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Keywords

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