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The antitumor effect of folic acid conjugated-Auricularia auricular polysaccharide-cisplatin complex on cervical carcinoma cells in nude mice

  • Junqiang Qiu
  • , Hua Zhang
  • , Zhenyu Wang*
  • , Didi Liu
  • , Shumin Liu
  • , Wei Han
  • , Joe M. Regenstein
  • , Lin Geng
  • *Corresponding author for this work
  • School of Chemistry and Chemical Engineering, Harbin Institute of Technology
  • Heilongjiang University of Traditional Chinese Medicine
  • Cornell University
  • Harbin Institute of Technology

Research output: Contribution to journalArticlepeer-review

Abstract

A tumor-targeted, folic acid (FA) conjugated-Auricularia auricular polysaccharide (AAP) -cis-diaminedichloroplatinum (CDDP) complex (FA-AAP-CDDP) was used for cervical carcinoma chemotherapy. The drug delivery system was able to enhance the antitumor potency of CDDP, and to reduce the toxic side effects of CDDP. The kidney of mice treated by FA-AAP-CDDP complex had higher superoxide dismutase, catalase, and glutathione peroxidase activities, and lower malondialdehyde. FA-AAP-CDDP complex could induce more interleukin-2, interleukin-4, and interferon-γ in mice. In addition, the FA-AAP-CDDP complex significantly promoted the expression of Bax and caspase-3 protein, but inhibited the expression of Bcl-2 protein, which activated the mitochondrial apoptotic pathway of tumor cells in nude mice. Moreover, the FA-AAP-CDDP complex had a higher intratumoral accumulation, was lower in the kidneys. This study may provide a new direction for folate receptor targeted polymers to improve anti-tumor activity, but reduce side effects of CDDP.

Original languageEnglish
Pages (from-to)2180-2189
Number of pages10
JournalInternational Journal of Biological Macromolecules
Volume107
DOIs
StatePublished - Feb 2018
Externally publishedYes

Keywords

  • Cisplatin
  • Folate receptors
  • Folic acid
  • Nude mice
  • Tumor cells

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