The aniline purifying efficiency of a combined mycelial pellet

The goals of this research were twofold; to avoid loss of bacterial agents, maintaining the concentration of bacteria degrading aniline in a system, and to improve efficiency when purifying aniline. Tests were done to determine the purifying efficiency of mycelial pellets working as a biomass carrier stabilizing engineered bacteria. The effects of aniline biodegradation were studied for mycelial pellets made from a combination of the mold Y3 and the bacteria JH-9. Purifying efficiencies were compared between the combined mycelial pellet and the bacteria JH-9. The purifying efficiency of the combined mycelial pellet was influenced by its inoculation ratio and the influent concentration of aniline. This was tested in a batch reactor. The results showed that the best inoculation ratio was 1mL fungal spore suspension of Y3 (A₆₂₀=0.25) with 3 mL bacterial suspension of JH-9 (bacteria concentration: 1.2× 10⁹ cfu/mL). Aniline concentrations of less than 500 mg/L did not cause inhibition for the combined mycelial pellet. The effluent of the batch reactor was stable. Aniline was completely eliminated and the chemical oxygen demand (COD) was reduced 60%.