Synergistic effect of hydrogen and nanoscale zero-valent iron on ex-situ biogas upgrading and acetate recovery

Hydrogen (H₂) assisted ex-situ biogas upgrading and liquid chemicals production can augment the fossil fuel-dominated energy market, and alleviate CO₂-induced global warming. Recent investigations confirmed that nanoscale zero-valent iron (nZVI) enabled the enhancement of anaerobic digestion for biogas production. However, little is known about the effect of nZVI on the downstream ex-situ biogas upgrading. Herein, different levels (0 mg L⁻¹, 100 mg L⁻¹, 200 mg L⁻¹, 500 mg L⁻¹, 1000 mg L⁻¹, 2000 mg L⁻¹) of nZVI were added for H₂-assisted ex-situ biogas upgrading, to study whether nZVI could impact the biomethane purity and acetate yield for the first time. Results showed that all tested nZVI levels were favorable for biogas upgrading in the presence of H₂, the highest biomethane content (94.1 %, v/v), the CO₂ utilization ratio (95.9 %), and acetate yield (19.4 mmol L⁻¹) were achieved at 500 mg L⁻¹ nZVI, respectively. Further analysis indicated that increased biogas upgrading efficiency was related to an increase in extracellular polymeric substances, which ensures the microbial activity and stability of the ex-situ biogas upgrading. Microbial community characterization showed that the Petrimonas, Romboutsia, Acidaminococcus, and Clostridium predominated the microbiome during biogas upgrading at 500 mg L⁻¹ nZVI with H₂ supply. These results suggested that nZVI and H₂ contributed jointly to promoting the bioconversion of CO₂ in biogas to acetate. The findings could be helpful for paving a new way for efficient simultaneous ex-situ biogas upgrading and liquid chemicals recovery.