Abstract
We observed the ultrastructure of nucleolus in rat liver cells by conventional electron microscopy, and employed cytochemistry NAMA-Ur DNA specific stain method to analyze the distribution and position of nucleolar DNA in situ. The results showed that nucleolar DNA of rat liver cells comes from nucleolus-associated chromatin, and continuously extends in the dense fibrillar component (DFC) of nucleolus, localizes at the periphery of fibrillar center (FC) and in DFC. Furthermore, by employing anti-DNA/RNA hybrid antibodies, we directly and selectively labeled transcription sites of rRNA genes and testified that localization of transcription sites not only to DFC but also to the periphery of FC.
| Original language | English |
|---|---|
| Pages (from-to) | 302-309 |
| Number of pages | 8 |
| Journal | Science in China, Series C: Life Sciences |
| Volume | 43 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2000 |
| Externally published | Yes |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 7 Affordable and Clean Energy
Keywords
- Location of nucleolar DNA in situ
- NAMA-Ur method
- Nucleolus
- Rat liver cell
- Transcription sites of rRNA genes
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