Structure and hypoglycemic effect of a neutral polysaccharide isolated from sea cucumber Stichopus japonicus

Pi Xian Gong; Yan Chao Wu; Ying Liu; Shi Zhong Lv; Yue You; Ze Lin Zhou; Xi Chen; Hui Jing Li

doi:10.1016/j.ijbiomac.2022.06.160

Structure and hypoglycemic effect of a neutral polysaccharide isolated from sea cucumber Stichopus japonicus

Pi Xian Gong
, Yan Chao Wu
, Ying Liu^*
, Shi Zhong Lv
, Yue You
, Ze Lin Zhou
, Xi Chen
, Hui Jing Li^*

^*Corresponding author for this work

Harbin Institute of Technology Weihai
Ltd.

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

In addition to its high nutritious value, sea cucumber has been recognized by folk medicine for a long time. This study investigated the structure and hyperglycemic activity of a neutral polysaccharide (NPsj) from sea cucumber Stichopus japonicus, whose molecular weight was determined as 301.75 kDa by HPGPC method. Monosaccharide composition analysis indicated that NPsj is a glucan. The structure of NPsj was obtained by combining the analysis of methylation analysis, FTIR, NMR, periodate oxidation, Smith degradation and ESI-MS, which is mainly composed of (1 → 4)-α-D-glucoses with β-D-glucose(1→) branches substituted at O-6 every 7–9 of 1,4 linked glucoses. An in vitro insulin resistance Hep G2 cells model and a 3 T3-L1 cells model were established, and the NPsj has significant effect to increase glucose consumption with no toxicity at 10–100 μg/mL. Furthermore, NPsj upregulates the phosphorylation of Akt1 and down-regulated GSK3β, and then reduces the phosphorylation of GS, indicating its mechanism of ameliorating insulin resistance via Akt/GSK3β/GS signaling pathway.

Original language	English
Pages (from-to)	14-23
Number of pages	10
Journal	International Journal of Biological Macromolecules
Volume	216
DOIs	https://doi.org/10.1016/j.ijbiomac.2022.06.160
State	Published - 1 Sep 2022
Externally published	Yes

Keywords

Antidiabetic
Glucan
Stichopus japonicus

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10.1016/j.ijbiomac.2022.06.160

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title = "Structure and hypoglycemic effect of a neutral polysaccharide isolated from sea cucumber Stichopus japonicus",

abstract = "In addition to its high nutritious value, sea cucumber has been recognized by folk medicine for a long time. This study investigated the structure and hyperglycemic activity of a neutral polysaccharide (NPsj) from sea cucumber Stichopus japonicus, whose molecular weight was determined as 301.75 kDa by HPGPC method. Monosaccharide composition analysis indicated that NPsj is a glucan. The structure of NPsj was obtained by combining the analysis of methylation analysis, FTIR, NMR, periodate oxidation, Smith degradation and ESI-MS, which is mainly composed of (1 → 4)-α-D-glucoses with β-D-glucose(1→) branches substituted at O-6 every 7–9 of 1,4 linked glucoses. An in vitro insulin resistance Hep G2 cells model and a 3 T3-L1 cells model were established, and the NPsj has significant effect to increase glucose consumption with no toxicity at 10–100 μg/mL. Furthermore, NPsj upregulates the phosphorylation of Akt1 and down-regulated GSK3β, and then reduces the phosphorylation of GS, indicating its mechanism of ameliorating insulin resistance via Akt/GSK3β/GS signaling pathway.",

keywords = "Antidiabetic, Glucan, Stichopus japonicus",

author = "Gong, \{Pi Xian\} and Wu, \{Yan Chao\} and Ying Liu and Lv, \{Shi Zhong\} and Yue You and Zhou, \{Ze Lin\} and Xi Chen and Li, \{Hui Jing\}",

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year = "2022",

month = sep,

day = "1",

doi = "10.1016/j.ijbiomac.2022.06.160",

language = "英语",

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T1 - Structure and hypoglycemic effect of a neutral polysaccharide isolated from sea cucumber Stichopus japonicus

AU - Gong, Pi Xian

AU - Wu, Yan Chao

AU - Liu, Ying

AU - Lv, Shi Zhong

AU - You, Yue

AU - Zhou, Ze Lin

AU - Chen, Xi

AU - Li, Hui Jing

PY - 2022/9/1

Y1 - 2022/9/1

N2 - In addition to its high nutritious value, sea cucumber has been recognized by folk medicine for a long time. This study investigated the structure and hyperglycemic activity of a neutral polysaccharide (NPsj) from sea cucumber Stichopus japonicus, whose molecular weight was determined as 301.75 kDa by HPGPC method. Monosaccharide composition analysis indicated that NPsj is a glucan. The structure of NPsj was obtained by combining the analysis of methylation analysis, FTIR, NMR, periodate oxidation, Smith degradation and ESI-MS, which is mainly composed of (1 → 4)-α-D-glucoses with β-D-glucose(1→) branches substituted at O-6 every 7–9 of 1,4 linked glucoses. An in vitro insulin resistance Hep G2 cells model and a 3 T3-L1 cells model were established, and the NPsj has significant effect to increase glucose consumption with no toxicity at 10–100 μg/mL. Furthermore, NPsj upregulates the phosphorylation of Akt1 and down-regulated GSK3β, and then reduces the phosphorylation of GS, indicating its mechanism of ameliorating insulin resistance via Akt/GSK3β/GS signaling pathway.

AB - In addition to its high nutritious value, sea cucumber has been recognized by folk medicine for a long time. This study investigated the structure and hyperglycemic activity of a neutral polysaccharide (NPsj) from sea cucumber Stichopus japonicus, whose molecular weight was determined as 301.75 kDa by HPGPC method. Monosaccharide composition analysis indicated that NPsj is a glucan. The structure of NPsj was obtained by combining the analysis of methylation analysis, FTIR, NMR, periodate oxidation, Smith degradation and ESI-MS, which is mainly composed of (1 → 4)-α-D-glucoses with β-D-glucose(1→) branches substituted at O-6 every 7–9 of 1,4 linked glucoses. An in vitro insulin resistance Hep G2 cells model and a 3 T3-L1 cells model were established, and the NPsj has significant effect to increase glucose consumption with no toxicity at 10–100 μg/mL. Furthermore, NPsj upregulates the phosphorylation of Akt1 and down-regulated GSK3β, and then reduces the phosphorylation of GS, indicating its mechanism of ameliorating insulin resistance via Akt/GSK3β/GS signaling pathway.

KW - Antidiabetic

KW - Glucan

KW - Stichopus japonicus

UR - https://www.scopus.com/pages/publications/85133236204

U2 - 10.1016/j.ijbiomac.2022.06.160

DO - 10.1016/j.ijbiomac.2022.06.160

M3 - 文章

C2 - 35780917

AN - SCOPUS:85133236204

SN - 0141-8130

VL - 216

SP - 14

EP - 23

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Structure and hypoglycemic effect of a neutral polysaccharide isolated from sea cucumber Stichopus japonicus

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Keywords

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