Simultaneous chromium removal and lipid accumulation by microalgae under acidic and low temperature conditions for promising biodiesel production

Microalgae have become the hotspot of recent researches as heavy metals (HMs) adsorbent and biodiesel production feedstock. In this study, the cell growth, lipid production and Cr⁶⁺ removal of Parachlorella kessleri R-3 at pH 3.5 and 15 °C were investigated. It was found that low concentration of Cr⁶⁺ (0.5 to 2 mg/L) promoted the algal growth, whereas Cr⁶⁺ higher than 5 mg/L inhibited the growth of P. kessleri R-3. Biomass concentration (2.40 g/L) and lipid productivity (131.79 mg/L d⁻¹) reached the highest at 2 mg/L Cr⁶⁺, and lipid content (61.03 %) reached the highest at 5 mg/L Cr⁶⁺. The maximum Cr⁶⁺ removal efficiency of 91 % was obtained at 0.5 mg/L Cr⁶⁺ treatment. Furthermore, fatty acid composition analysis showed that strain R-3 had a high C16-18 content of 74.88–89.21 %. This study provides new insight into the treatment of HMs and lipid production in cold regions.