Sessile methanogens dominated cathodic biofilm: Distribution and network in physiological transitions

A mature cathodic biofilm plays the key role in biocathode electron transfer. The physiological transitions of it were of great interests as the sessile and dispersed (planktonic) work in a balance while it is still lack of understanding. In this study, an improved sampling method was used for detecting sessile, detached, and dispersed microorganisms. The results indicated a certain number of methanogens (82.6%) and its associated phyla (60%–90%) are immobilized as sessile biofilm. The Tax4Fun predicted a lower abundance of mobility associated genes and a significant enrichment (t-test, P = 0.003) of c-di-GMP in sessile biofilm. Overall, the microbial interaction and motility were predicted as two factors to affect the physiological transitions of cathodic biofilm. This finding could shed a light on the investigation of cathodic biofilm in a dynamic transition rather than a static community, playing a pivotal role in understanding the relation between specific property of biofilm.