Screening of bile salt hydrolase-active lactic acid bacteria for potential cholesterol-lowering probiotic use

Chunfeng Guo; Lanwei Zhang; Jingyan Li; Yingchun Zhang; Chaohui Xue; Huaxi Yi; Ming Du; Xue Han

doi:10.4028/www.scientific.net/AMR.345.139

Screening of bile salt hydrolase-active lactic acid bacteria for potential cholesterol-lowering probiotic use

Chunfeng Guo
, Lanwei Zhang^*
, Jingyan Li
, Yingchun Zhang
, Chaohui Xue
, Huaxi Yi
, Ming Du
, Xue Han

^*Corresponding author for this work

Harbin Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

7 Scopus citations

Abstract

Cholesterol-lowering effect of lactic acid bacteria (LAB) with bile salt hydrolase activity is well known. In this study, 150 LAB were screened for bile salt deconjugation ability and probiotic characters. Fourteen isolates with higher bile salt deconjugation ability were initially screened out using deconjugation rate above 50% as standard. These isolates were further screened for adhesion to HT-29 cells, bile tolerance and acid resistance. Four isolates, namely Lactobacillus casei F0822, Lactobacillus casei F0422, Enterococcus faecium F0511 and Enterococcus faecium IN7.12, was finally screened out. The 4 isolates may be able to reduce serum cholesterol levels in human and thus have a potential to apply in the biomedicine field.

Original language	English
Title of host publication	Smart Materials and Nanotechnology in Engineering, SMNE2011
Pages	139-146
Number of pages	8
DOIs	https://doi.org/10.4028/www.scientific.net/AMR.345.139
State	Published - 2012
Event	2011 International Conference on Smart Materials and Nanotechnology in Engineering, SMNE 2011 - Wuhan, China Duration: 17 Sep 2011 → 18 Sep 2011

Publication series

Name	Advanced Materials Research
Volume	345
ISSN (Print)	1022-6680

Conference

Conference	2011 International Conference on Smart Materials and Nanotechnology in Engineering, SMNE 2011
Country/Territory	China
City	Wuhan
Period	17/09/11 → 18/09/11

Keywords

Bile deconjugation
Cholesterol-lowering
Probiotic

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10.4028/www.scientific.net/AMR.345.139

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@inproceedings{b02baf2bc290485abe4aa69d2ca12213,

title = "Screening of bile salt hydrolase-active lactic acid bacteria for potential cholesterol-lowering probiotic use",

abstract = "Cholesterol-lowering effect of lactic acid bacteria (LAB) with bile salt hydrolase activity is well known. In this study, 150 LAB were screened for bile salt deconjugation ability and probiotic characters. Fourteen isolates with higher bile salt deconjugation ability were initially screened out using deconjugation rate above 50\% as standard. These isolates were further screened for adhesion to HT-29 cells, bile tolerance and acid resistance. Four isolates, namely Lactobacillus casei F0822, Lactobacillus casei F0422, Enterococcus faecium F0511 and Enterococcus faecium IN7.12, was finally screened out. The 4 isolates may be able to reduce serum cholesterol levels in human and thus have a potential to apply in the biomedicine field.",

keywords = "Bile deconjugation, Cholesterol-lowering, Probiotic",

author = "Chunfeng Guo and Lanwei Zhang and Jingyan Li and Yingchun Zhang and Chaohui Xue and Huaxi Yi and Ming Du and Xue Han",

year = "2012",

doi = "10.4028/www.scientific.net/AMR.345.139",

language = "英语",

isbn = "9783037852576",

series = "Advanced Materials Research",

pages = "139--146",

booktitle = "Smart Materials and Nanotechnology in Engineering, SMNE2011",

note = "2011 International Conference on Smart Materials and Nanotechnology in Engineering, SMNE 2011 ; Conference date: 17-09-2011 Through 18-09-2011",

}

Guo, C, Zhang, L, Li, J, Zhang, Y, Xue, C, Yi, H, Du, M & Han, X 2012, Screening of bile salt hydrolase-active lactic acid bacteria for potential cholesterol-lowering probiotic use. in Smart Materials and Nanotechnology in Engineering, SMNE2011. Advanced Materials Research, vol. 345, pp. 139-146, 2011 International Conference on Smart Materials and Nanotechnology in Engineering, SMNE 2011, Wuhan, China, 17/09/11. https://doi.org/10.4028/www.scientific.net/AMR.345.139

Screening of bile salt hydrolase-active lactic acid bacteria for potential cholesterol-lowering probiotic use. / Guo, Chunfeng; Zhang, Lanwei; Li, Jingyan et al.
Smart Materials and Nanotechnology in Engineering, SMNE2011. 2012. p. 139-146 (Advanced Materials Research; Vol. 345).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Screening of bile salt hydrolase-active lactic acid bacteria for potential cholesterol-lowering probiotic use

AU - Guo, Chunfeng

AU - Zhang, Lanwei

AU - Li, Jingyan

AU - Zhang, Yingchun

AU - Xue, Chaohui

AU - Yi, Huaxi

AU - Du, Ming

AU - Han, Xue

PY - 2012

Y1 - 2012

N2 - Cholesterol-lowering effect of lactic acid bacteria (LAB) with bile salt hydrolase activity is well known. In this study, 150 LAB were screened for bile salt deconjugation ability and probiotic characters. Fourteen isolates with higher bile salt deconjugation ability were initially screened out using deconjugation rate above 50% as standard. These isolates were further screened for adhesion to HT-29 cells, bile tolerance and acid resistance. Four isolates, namely Lactobacillus casei F0822, Lactobacillus casei F0422, Enterococcus faecium F0511 and Enterococcus faecium IN7.12, was finally screened out. The 4 isolates may be able to reduce serum cholesterol levels in human and thus have a potential to apply in the biomedicine field.

AB - Cholesterol-lowering effect of lactic acid bacteria (LAB) with bile salt hydrolase activity is well known. In this study, 150 LAB were screened for bile salt deconjugation ability and probiotic characters. Fourteen isolates with higher bile salt deconjugation ability were initially screened out using deconjugation rate above 50% as standard. These isolates were further screened for adhesion to HT-29 cells, bile tolerance and acid resistance. Four isolates, namely Lactobacillus casei F0822, Lactobacillus casei F0422, Enterococcus faecium F0511 and Enterococcus faecium IN7.12, was finally screened out. The 4 isolates may be able to reduce serum cholesterol levels in human and thus have a potential to apply in the biomedicine field.

KW - Bile deconjugation

KW - Cholesterol-lowering

KW - Probiotic

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DO - 10.4028/www.scientific.net/AMR.345.139

M3 - 会议稿件

AN - SCOPUS:80053593249

SN - 9783037852576

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BT - Smart Materials and Nanotechnology in Engineering, SMNE2011

T2 - 2011 International Conference on Smart Materials and Nanotechnology in Engineering, SMNE 2011

Y2 - 17 September 2011 through 18 September 2011

ER -

Screening of bile salt hydrolase-active lactic acid bacteria for potential cholesterol-lowering probiotic use

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