TY - CHAP
T1 - Robotic Microinjection of Zebrafish Larva
AU - Zhuang, Songlin
AU - Zhang, Gefei
AU - Lei, Dongxu
AU - Yu, Xinghu
AU - Tong, Mingsi
AU - Lin, Weiyang
AU - Shi, Yang
AU - Gao, Huijun
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2023
Y1 - 2023
N2 - Autonomous drug delivery to some organs inside a small living organism is crucial for understanding individual-level responses, testing drug toxicity, and developing new therapeutic approaches. However, in vivo microinjection requires a series of complex operations on delicate and highly deformable vertebrate models. Here we develop an autonomous drug delivery system that can perform multi-axis orientation, dynamic immobilization, and adaptive heart microinjection for zebrafish larvae. System performances including orientation and immobilization accuracy, success rate, and operation speed were quantitatively evaluated. Experimental results showed that the robotic system achieved orientation accuracies of 5 o and 3.6 o, respectively about the dorsal-ventral axis and the anterior-posterior axis of zebrafish, in aligning zebrafish heart to an injection pipette tip, and the yolk deformation accuracy of 1.7 μ m in zebrafish immobilization. The success rate of the autonomous drug delivery was 92.3%, and the cost time for one zebrafish operation was 60–70 s. This chapter also reports the delivery efficiency of one cardiotoxic drug (verapamil hydrochloride) and one fluorescent nanomaterial (fluorescence carbon dots) among three delivery methods (direct soaking, yolk sac microinjection, and heart microinjection) using three specific phenotypic endpoints (heart rate, pericardial edema, and fluorescent distribution).
AB - Autonomous drug delivery to some organs inside a small living organism is crucial for understanding individual-level responses, testing drug toxicity, and developing new therapeutic approaches. However, in vivo microinjection requires a series of complex operations on delicate and highly deformable vertebrate models. Here we develop an autonomous drug delivery system that can perform multi-axis orientation, dynamic immobilization, and adaptive heart microinjection for zebrafish larvae. System performances including orientation and immobilization accuracy, success rate, and operation speed were quantitatively evaluated. Experimental results showed that the robotic system achieved orientation accuracies of 5 o and 3.6 o, respectively about the dorsal-ventral axis and the anterior-posterior axis of zebrafish, in aligning zebrafish heart to an injection pipette tip, and the yolk deformation accuracy of 1.7 μ m in zebrafish immobilization. The success rate of the autonomous drug delivery was 92.3%, and the cost time for one zebrafish operation was 60–70 s. This chapter also reports the delivery efficiency of one cardiotoxic drug (verapamil hydrochloride) and one fluorescent nanomaterial (fluorescence carbon dots) among three delivery methods (direct soaking, yolk sac microinjection, and heart microinjection) using three specific phenotypic endpoints (heart rate, pericardial edema, and fluorescent distribution).
UR - https://www.scopus.com/pages/publications/85163693655
U2 - 10.1007/978-3-031-33410-8_6
DO - 10.1007/978-3-031-33410-8_6
M3 - 章节
AN - SCOPUS:85163693655
T3 - Synthesis Lectures on Biomedical Engineering
SP - 167
EP - 186
BT - Synthesis Lectures on Biomedical Engineering
PB - Springer Nature
ER -