Recombinant luminal domain of human synaptotagmin II in combination with gangliosides inhibits the toxicity of botulinum neurotoxins in mice

Jing Shi; Tao Li; Xiaojun Hou; Kun Cai; Shizhong Bao; Hao Liu; Xiang Gao; Le Xiao; Wei Tu; Qin Wang; Jun Yin; Hui Wang

doi:10.1016/j.micinf.2010.01.002

Recombinant luminal domain of human synaptotagmin II in combination with gangliosides inhibits the toxicity of botulinum neurotoxins in mice

Jing Shi
, Tao Li
, Xiaojun Hou
, Kun Cai
, Shizhong Bao
, Hao Liu
, Xiang Gao
, Le Xiao
, Wei Tu
, Qin Wang
, Jun Yin
, Hui Wang^*

^*Corresponding author for this work

Academy of Military Medical Science China

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Synaptotagmin II (syt II) is the specific protein receptor of botulinum neurotoxin B (BoNT/B), and the luminal domain of syt II contains toxin-binding sites that have a high affinity for BoNT/B. However, it is not yet clear whether the luminal domain of syt II (syt II-LD) inhibits the toxicity of BoNT/B by interfering with the toxin-receptor interaction. In this study, we characterized the binding of the purified recombinant syt II-LD to BoNT and revealed that the recombinant syt II-LD in vivo could provide protection against BoNT/B intoxication in mice models, and the neutralization effect could be improved by using gangliosides.

Original language	English
Pages (from-to)	319-323
Number of pages	5
Journal	Microbes and Infection
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/j.micinf.2010.01.002
State	Published - Apr 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Botulinum neurotoxin
Ganglioside
Receptor
Synaptotagmin

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10.1016/j.micinf.2010.01.002

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title = "Recombinant luminal domain of human synaptotagmin II in combination with gangliosides inhibits the toxicity of botulinum neurotoxins in mice",

abstract = "Synaptotagmin II (syt II) is the specific protein receptor of botulinum neurotoxin B (BoNT/B), and the luminal domain of syt II contains toxin-binding sites that have a high affinity for BoNT/B. However, it is not yet clear whether the luminal domain of syt II (syt II-LD) inhibits the toxicity of BoNT/B by interfering with the toxin-receptor interaction. In this study, we characterized the binding of the purified recombinant syt II-LD to BoNT and revealed that the recombinant syt II-LD in vivo could provide protection against BoNT/B intoxication in mice models, and the neutralization effect could be improved by using gangliosides.",

keywords = "Botulinum neurotoxin, Ganglioside, Receptor, Synaptotagmin",

author = "Jing Shi and Tao Li and Xiaojun Hou and Kun Cai and Shizhong Bao and Hao Liu and Xiang Gao and Le Xiao and Wei Tu and Qin Wang and Jun Yin and Hui Wang",

year = "2010",

month = apr,

doi = "10.1016/j.micinf.2010.01.002",

language = "英语",

volume = "12",

pages = "319--323",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier Masson s.r.l.",

number = "4",

}

TY - JOUR

T1 - Recombinant luminal domain of human synaptotagmin II in combination with gangliosides inhibits the toxicity of botulinum neurotoxins in mice

AU - Shi, Jing

AU - Li, Tao

AU - Hou, Xiaojun

AU - Cai, Kun

AU - Bao, Shizhong

AU - Liu, Hao

AU - Gao, Xiang

AU - Xiao, Le

AU - Tu, Wei

AU - Wang, Qin

AU - Yin, Jun

AU - Wang, Hui

PY - 2010/4

Y1 - 2010/4

N2 - Synaptotagmin II (syt II) is the specific protein receptor of botulinum neurotoxin B (BoNT/B), and the luminal domain of syt II contains toxin-binding sites that have a high affinity for BoNT/B. However, it is not yet clear whether the luminal domain of syt II (syt II-LD) inhibits the toxicity of BoNT/B by interfering with the toxin-receptor interaction. In this study, we characterized the binding of the purified recombinant syt II-LD to BoNT and revealed that the recombinant syt II-LD in vivo could provide protection against BoNT/B intoxication in mice models, and the neutralization effect could be improved by using gangliosides.

AB - Synaptotagmin II (syt II) is the specific protein receptor of botulinum neurotoxin B (BoNT/B), and the luminal domain of syt II contains toxin-binding sites that have a high affinity for BoNT/B. However, it is not yet clear whether the luminal domain of syt II (syt II-LD) inhibits the toxicity of BoNT/B by interfering with the toxin-receptor interaction. In this study, we characterized the binding of the purified recombinant syt II-LD to BoNT and revealed that the recombinant syt II-LD in vivo could provide protection against BoNT/B intoxication in mice models, and the neutralization effect could be improved by using gangliosides.

KW - Botulinum neurotoxin

KW - Ganglioside

KW - Receptor

KW - Synaptotagmin

UR - https://www.scopus.com/pages/publications/77949261489

U2 - 10.1016/j.micinf.2010.01.002

DO - 10.1016/j.micinf.2010.01.002

M3 - 文章

C2 - 20080203

AN - SCOPUS:77949261489

SN - 1286-4579

VL - 12

SP - 319

EP - 323

JO - Microbes and Infection

JF - Microbes and Infection

IS - 4

ER -

Recombinant luminal domain of human synaptotagmin II in combination with gangliosides inhibits the toxicity of botulinum neurotoxins in mice

Abstract

UN SDGs

Keywords

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