TY - GEN
T1 - Radiation protection of carboxymethylation of an acid polysaccharides extracted from auricularia auricula against Uvb in vitro
AU - Zhang, Hua
AU - Wang, Zhen Yu
AU - Fan, Zi Luan
AU - Yang, Xin
AU - Wang, Xue
AU - Zhang, Ning
PY - 2012
Y1 - 2012
N2 - This paper reports on a water-soluble acid polysaccharide (AAP) and in how it was extracted from Auricularia auricular, acquired by CTAB, and prepared it's carboxymethylation. Chemical characterization by high-performance liquid chromatography/gel permeation chromatograph (HPLC/GPC), Fourier transform infrared (FT-IR) spectrometer and gas chromatography-mass spectrophotometer (GC-MS) were investigated. Chemical analysis indicated that C AAAP was composed of arabinose, xylose, mannose, glucose and galactose, with the molar ratio at 0.04: 0.13: 1.00: 0.59: 0.29. Moreover, radiation protection against UVB in vitro indicated that at the dose range of 200-500 μg/mL, C AAAP enhanced the protection of HepG2 cells against UVB cytotoxicity than AAAP. However, but at the dose range of 50-150 μg/mL the result was just opposite.
AB - This paper reports on a water-soluble acid polysaccharide (AAP) and in how it was extracted from Auricularia auricular, acquired by CTAB, and prepared it's carboxymethylation. Chemical characterization by high-performance liquid chromatography/gel permeation chromatograph (HPLC/GPC), Fourier transform infrared (FT-IR) spectrometer and gas chromatography-mass spectrophotometer (GC-MS) were investigated. Chemical analysis indicated that C AAAP was composed of arabinose, xylose, mannose, glucose and galactose, with the molar ratio at 0.04: 0.13: 1.00: 0.59: 0.29. Moreover, radiation protection against UVB in vitro indicated that at the dose range of 200-500 μg/mL, C AAAP enhanced the protection of HepG2 cells against UVB cytotoxicity than AAAP. However, but at the dose range of 50-150 μg/mL the result was just opposite.
KW - Acid auricularia auricula polysaccharide(AAAP)
KW - Carboxymethylation acid auricularia auricula polysaccharide(C AAAP)
KW - In vitro
KW - Radiation protection
KW - UVB
UR - https://www.scopus.com/pages/publications/81055133545
U2 - 10.4028/www.scientific.net/AMR.365.199
DO - 10.4028/www.scientific.net/AMR.365.199
M3 - 会议稿件
AN - SCOPUS:81055133545
SN - 9783037852712
T3 - Advanced Materials Research
SP - 199
EP - 204
BT - Future Materials Engineering and Industry Application
T2 - 2011 International Conference on Future Materials Engineering and Industry Application, ICFMEIA 2011
Y2 - 4 August 2011 through 5 August 2011
ER -