Abstract
Aim: To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion. Methods: The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber. Results: p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner. Conclusions: p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.
| Original language | English |
|---|---|
| Pages (from-to) | 499-506 |
| Number of pages | 8 |
| Journal | Acta Pharmacologica Sinica |
| Volume | 29 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2008 |
| Externally published | Yes |
Keywords
- Cell adhesion inhibition
- Inflammation
- P-selectin
- Polysaccharides of Ginkgo biloba leaves
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