Pseudomonas putida KT2442 as a platform for the biosynthesis of polyhydroxyalkanoates with adjustable monomer contents and compositions

Lakshmi Tripathi; Lin Ping Wu; Meng Dechuan; Jinchun Chen; Qiong Wu; Guo Qiang Chen

doi:10.1016/j.biortech.2013.05.027

Pseudomonas putida KT2442 as a platform for the biosynthesis of polyhydroxyalkanoates with adjustable monomer contents and compositions

Lakshmi Tripathi
, Lin Ping Wu
, Meng Dechuan
, Jinchun Chen
, Qiong Wu
, Guo Qiang Chen^*

^*Corresponding author for this work

Tsinghua University
University of Copenhagen

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

The β-oxidation weakened Pseudomonas putida were established as a platform for the production of polyhydroxyalkanoates (PHA) with adjustable monomer compositions and micro-structures. When mutant P. putida KTOYO6δC (phaPCJ_A.c) was cultivated on mixtures of sodium butyrate and sodium hexanoate (C4:C6), random copolymers of P(3HB-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyhexanoate (3HHx), were accumulated with 3HHx content ranged from 19mol% to 75mol%. While recombinant P. putida KTQQ20 grown on mixtures of sodium hexanoate and decanoic acid (C6:C10), produced random copolymers of P(3HHx-co-3HD) consisting of 3-hydroxyhexanoate (3HHx) and 3-hydroxydecanoate (3HD), the monomer fraction of 3HHx ranged from 16mol% to 63mol%. The comonomer compositions were easily regulated by varying the fatty acid concentrations. P. putida KTQQ20 produced a novel diblock copolymer P3HHx-b-P(3HD-co-3HDD) consisting of 49mol% P3HHx and 51mol% P(3HD-co-3HDD) [35.25mol% 3HDD (3-hydroxydodecanoate)], which was characterized by ¹³C NMR, HMBC NMR, DSC, GPC and universal testing machine.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	Bioresource Technology
Volume	142
DOIs	https://doi.org/10.1016/j.biortech.2013.05.027
State	Published - Aug 2013
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Block copolymer
Polyhydroxyalkanoate
Pseudomonas putida
Random copolymer

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10.1016/j.biortech.2013.05.027

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@article{9217679bd1634665ad67f50e6010cb0b,

title = "Pseudomonas putida KT2442 as a platform for the biosynthesis of polyhydroxyalkanoates with adjustable monomer contents and compositions",

abstract = "The β-oxidation weakened Pseudomonas putida were established as a platform for the production of polyhydroxyalkanoates (PHA) with adjustable monomer compositions and micro-structures. When mutant P. putida KTOYO6δC (phaPCJA.c) was cultivated on mixtures of sodium butyrate and sodium hexanoate (C4:C6), random copolymers of P(3HB-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyhexanoate (3HHx), were accumulated with 3HHx content ranged from 19mol\% to 75mol\%. While recombinant P. putida KTQQ20 grown on mixtures of sodium hexanoate and decanoic acid (C6:C10), produced random copolymers of P(3HHx-co-3HD) consisting of 3-hydroxyhexanoate (3HHx) and 3-hydroxydecanoate (3HD), the monomer fraction of 3HHx ranged from 16mol\% to 63mol\%. The comonomer compositions were easily regulated by varying the fatty acid concentrations. P. putida KTQQ20 produced a novel diblock copolymer P3HHx-b-P(3HD-co-3HDD) consisting of 49mol\% P3HHx and 51mol\% P(3HD-co-3HDD) [35.25mol\% 3HDD (3-hydroxydodecanoate)], which was characterized by 13C NMR, HMBC NMR, DSC, GPC and universal testing machine.",

keywords = "Block copolymer, Polyhydroxyalkanoate, Pseudomonas putida, Random copolymer",

author = "Lakshmi Tripathi and Wu, \{Lin Ping\} and Meng Dechuan and Jinchun Chen and Qiong Wu and Chen, \{Guo Qiang\}",

year = "2013",

month = aug,

doi = "10.1016/j.biortech.2013.05.027",

language = "英语",

volume = "142",

pages = "225--231",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Pseudomonas putida KT2442 as a platform for the biosynthesis of polyhydroxyalkanoates with adjustable monomer contents and compositions

AU - Tripathi, Lakshmi

AU - Wu, Lin Ping

AU - Dechuan, Meng

AU - Chen, Jinchun

AU - Wu, Qiong

AU - Chen, Guo Qiang

PY - 2013/8

Y1 - 2013/8

N2 - The β-oxidation weakened Pseudomonas putida were established as a platform for the production of polyhydroxyalkanoates (PHA) with adjustable monomer compositions and micro-structures. When mutant P. putida KTOYO6δC (phaPCJA.c) was cultivated on mixtures of sodium butyrate and sodium hexanoate (C4:C6), random copolymers of P(3HB-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyhexanoate (3HHx), were accumulated with 3HHx content ranged from 19mol% to 75mol%. While recombinant P. putida KTQQ20 grown on mixtures of sodium hexanoate and decanoic acid (C6:C10), produced random copolymers of P(3HHx-co-3HD) consisting of 3-hydroxyhexanoate (3HHx) and 3-hydroxydecanoate (3HD), the monomer fraction of 3HHx ranged from 16mol% to 63mol%. The comonomer compositions were easily regulated by varying the fatty acid concentrations. P. putida KTQQ20 produced a novel diblock copolymer P3HHx-b-P(3HD-co-3HDD) consisting of 49mol% P3HHx and 51mol% P(3HD-co-3HDD) [35.25mol% 3HDD (3-hydroxydodecanoate)], which was characterized by 13C NMR, HMBC NMR, DSC, GPC and universal testing machine.

AB - The β-oxidation weakened Pseudomonas putida were established as a platform for the production of polyhydroxyalkanoates (PHA) with adjustable monomer compositions and micro-structures. When mutant P. putida KTOYO6δC (phaPCJA.c) was cultivated on mixtures of sodium butyrate and sodium hexanoate (C4:C6), random copolymers of P(3HB-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyhexanoate (3HHx), were accumulated with 3HHx content ranged from 19mol% to 75mol%. While recombinant P. putida KTQQ20 grown on mixtures of sodium hexanoate and decanoic acid (C6:C10), produced random copolymers of P(3HHx-co-3HD) consisting of 3-hydroxyhexanoate (3HHx) and 3-hydroxydecanoate (3HD), the monomer fraction of 3HHx ranged from 16mol% to 63mol%. The comonomer compositions were easily regulated by varying the fatty acid concentrations. P. putida KTQQ20 produced a novel diblock copolymer P3HHx-b-P(3HD-co-3HDD) consisting of 49mol% P3HHx and 51mol% P(3HD-co-3HDD) [35.25mol% 3HDD (3-hydroxydodecanoate)], which was characterized by 13C NMR, HMBC NMR, DSC, GPC and universal testing machine.

KW - Block copolymer

KW - Polyhydroxyalkanoate

KW - Pseudomonas putida

KW - Random copolymer

UR - https://www.scopus.com/pages/publications/84878848014

U2 - 10.1016/j.biortech.2013.05.027

DO - 10.1016/j.biortech.2013.05.027

M3 - 文章

AN - SCOPUS:84878848014

SN - 0960-8524

VL - 142

SP - 225

EP - 231

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Pseudomonas putida KT2442 as a platform for the biosynthesis of polyhydroxyalkanoates with adjustable monomer contents and compositions

Abstract

UN SDGs

Keywords

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