Protective Role of Vitamin C in Wi-Fi Induced Oxidative Stress in MC3T3-E1 Cells in Vitro

The increasing convenience and benefits provided by wireless technology innovations may also affect the human health because of Wi-Fi electromagnetic radiation. The effects of 2.45 GHz Wi-Fi on oxidative stress (OS) in MC3T3-E1 cells and the protective role of vitamin C are presented and analyzed in this paper. MC3T3-E1 cells were exposed to 100 mW and 500 mW 2.45 GHz Wi-Fi signals at specific absorption rates (SARs) of 0.1671 W/kg and 0.8356 W/kg, referred to as SARa and SARb, respectively, for 0-180 minutes to determine the optimal irradiation time by testing reactive oxygen species (ROS) and glutathione (GSH). Following irradiation for the optimal irradiation time, ROS levels were assayed for 0-120 min after each irradiation. Additional vitamin C is added to the medium to investigate the effect on ROS and GSH. A FDTD simulation showed that the cell layer temperature increased by 0.1°C and 0.5°C after being exposed in the SARa and SARb for the optimal irradiation time (90 min). Ninety min of Wi-Fi irradiation provoked an obvious increment in ROS and GSH on the first day, and the ROS level returned to the initial level 30 min after the irradiation; however, on the third day, it took 90-120 min for ROS to return to baseline. Vitamin C significantly reduced ROS levels and recovery times. In conclusion, 2.45 GHz Wi-Fi radiation triggered oxidative stress in osteoblasts 3cm from the source antenna. Vitamin C effectively reduced the ROS levels stimulated by nonthermal effects of Wi-Fi irradiation.