Probiotic properties of Lacticaseibacillus rhamnosus grx10 revolved with complete genome

In present study, probiotic characteristics of Lacticaseibacillus rhamnosus grx10 was investigated by genotype and phenotype. In vitro experiments showed grx10 had excellent ability of resistance to ambient stresses (temperature, pH, and bile salt), bacteriostatic, and adhesion, which are necessary capabilities to be a potential probiotic. Besides, complete genome sequence of the strain showed basic genetic information and genes correspond to probiotic characteristics. Meanwhile, sequencie and analysis of complete genome to predict genes involved in extracellular proteins and their secretory pathway. Among the secreted proteins of grx10, found p40 and p75 which were shown to favor intestinal barrier integrity. In the fermented supernatant of grx10, the abundance of p40 and p75 was 0.36% and 0.20%, respectively. We expected that grx10 would be predicted to function to protect the intestinal barrier from postbiotics perspective.