Principles and Applications of Single Particle Tracking in Cell Research

Zhao Wang; Xuejing Wang; Ying Zhang; Weili Xu; Xiaojun Han

doi:10.1002/smll.202005133

Principles and Applications of Single Particle Tracking in Cell Research

Zhao Wang
, Xuejing Wang
, Ying Zhang
, Weili Xu
, Xiaojun Han^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering, Harbin Institute of Technology
Zhejiang University
Heilongjiang Institute of Technology

Research output: Contribution to journal › Review article › peer-review

27 Scopus citations

Abstract

It is a tough challenge for many decades to decipher the complex relationships between cell behaviors and cellular physical properties. Single particle tracking (SPT) with high spatial and temporal resolution has been applied extensively in cell research to understand physicochemical properties of cells and their bio-functions by tracking endogenous or exogenous probes. This review describes the fundamental principles of SPT as well as its applications in intracellular mechanics, membrane dynamics, organelles distribution, and processes of internalization and transport. Finally, challenges and future directions of SPT are also discussed.

Original language	English
Article number	2005133
Journal	Small
Volume	17
Issue number	11
DOIs	https://doi.org/10.1002/smll.202005133
State	Published - 18 Mar 2021
Externally published	Yes

Keywords

cell mechanics
intracellular transport
membrane dynamics
single particle tracking
trajectory analysis

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10.1002/smll.202005133

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abstract = "It is a tough challenge for many decades to decipher the complex relationships between cell behaviors and cellular physical properties. Single particle tracking (SPT) with high spatial and temporal resolution has been applied extensively in cell research to understand physicochemical properties of cells and their bio-functions by tracking endogenous or exogenous probes. This review describes the fundamental principles of SPT as well as its applications in intracellular mechanics, membrane dynamics, organelles distribution, and processes of internalization and transport. Finally, challenges and future directions of SPT are also discussed.",

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AU - Wang, Xuejing

AU - Zhang, Ying

AU - Xu, Weili

AU - Han, Xiaojun

PY - 2021/3/18

Y1 - 2021/3/18

N2 - It is a tough challenge for many decades to decipher the complex relationships between cell behaviors and cellular physical properties. Single particle tracking (SPT) with high spatial and temporal resolution has been applied extensively in cell research to understand physicochemical properties of cells and their bio-functions by tracking endogenous or exogenous probes. This review describes the fundamental principles of SPT as well as its applications in intracellular mechanics, membrane dynamics, organelles distribution, and processes of internalization and transport. Finally, challenges and future directions of SPT are also discussed.

AB - It is a tough challenge for many decades to decipher the complex relationships between cell behaviors and cellular physical properties. Single particle tracking (SPT) with high spatial and temporal resolution has been applied extensively in cell research to understand physicochemical properties of cells and their bio-functions by tracking endogenous or exogenous probes. This review describes the fundamental principles of SPT as well as its applications in intracellular mechanics, membrane dynamics, organelles distribution, and processes of internalization and transport. Finally, challenges and future directions of SPT are also discussed.

KW - cell mechanics

KW - intracellular transport

KW - membrane dynamics

KW - single particle tracking

KW - trajectory analysis

UR - https://www.scopus.com/pages/publications/85100208936

U2 - 10.1002/smll.202005133

DO - 10.1002/smll.202005133

M3 - 文献综述

C2 - 33533163

AN - SCOPUS:85100208936

SN - 1613-6810

VL - 17

JO - Small

JF - Small

IS - 11

M1 - 2005133

ER -

Principles and Applications of Single Particle Tracking in Cell Research

Abstract

Keywords

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