TY - GEN
T1 - Preparation of biomembrane by immobilizing enriched ammonia-oxidizing bacteria (AOB) in polyvinyl alcohol (PVA) for biosensor
AU - Cui, Rong
AU - Li, Jinling
AU - Zhang, Ke
AU - Han, Jinglong
PY - 2011
Y1 - 2011
N2 - A biomembrane that was used to make a biosensor for detecting inhibitor of ammoia oxidation was prepared by entrapping enriched ammonia-oxidizing bacteria (AOB) in polyvinyl alcohol (PVA). AOB were enriched by fed batch cultivation with using return activated sludge from A2/O process as seed. Enrichment of AOB was confirmed by denaturing gradient gel electrophoresis (DGGE) analysis. The optimum conditions based on orthogonal test results for biomembrane preparation were found as follows: mixing 30%(w/w) enriched cell with membrane matrix (12% (w/v) PVA and 3% (w/v) sodium alginate(SA), pouring mixture on a petri dish, dried for 1 hr, and solidified by soaking this membrane in a cross-linking agent (25% (w/v) NaNO3 and 2% (w/v) CaCl 2) for 1 h. A biosensor was fabricated by attaching the prepared biomembrane on an oxygen electrode. The bioactivity of prepared biosensor which was measured as DO drop was 1.4 mgO2/l, shows that PVA and SA can be used to immobilize AOB to produce a biosensor for detecting inhibitor of ammoia oxidation.
AB - A biomembrane that was used to make a biosensor for detecting inhibitor of ammoia oxidation was prepared by entrapping enriched ammonia-oxidizing bacteria (AOB) in polyvinyl alcohol (PVA). AOB were enriched by fed batch cultivation with using return activated sludge from A2/O process as seed. Enrichment of AOB was confirmed by denaturing gradient gel electrophoresis (DGGE) analysis. The optimum conditions based on orthogonal test results for biomembrane preparation were found as follows: mixing 30%(w/w) enriched cell with membrane matrix (12% (w/v) PVA and 3% (w/v) sodium alginate(SA), pouring mixture on a petri dish, dried for 1 hr, and solidified by soaking this membrane in a cross-linking agent (25% (w/v) NaNO3 and 2% (w/v) CaCl 2) for 1 h. A biosensor was fabricated by attaching the prepared biomembrane on an oxygen electrode. The bioactivity of prepared biosensor which was measured as DO drop was 1.4 mgO2/l, shows that PVA and SA can be used to immobilize AOB to produce a biosensor for detecting inhibitor of ammoia oxidation.
KW - Biosensor
KW - Cross link
KW - Enriched ammonia oxidizing bacteria
KW - Polyvinyl alcohol (PVA) membrane
UR - https://www.scopus.com/pages/publications/80052206734
U2 - 10.1109/RSETE.2011.5965483
DO - 10.1109/RSETE.2011.5965483
M3 - 会议稿件
AN - SCOPUS:80052206734
SN - 9781424491711
T3 - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings
SP - 5185
EP - 5188
BT - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings
T2 - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011
Y2 - 24 June 2011 through 26 June 2011
ER -