Abstract
Applying the Hungate anaerobic technology, 8 strains of sulfate reducing bacteria (SRB) were isolated from the wastewater of Daqing Oilfield. Terminal products were analyzed by gas chromatography and the sequence cloning of 16S-23S rDNA intergenic spacer region was used for the phylogenetic analysis of sulfate reducing bacteria. The results show that 8 strains of SRB belong to Salmonella D2 and Salmonella I7, Clostridium F4, Qostridium D10 and Clostridium H1, Anaerofilum A8 and Anaerofilum A18, and Enterobacter E1. The analysis of clustering shows that the length diversity for the intergenic spacer region sequence of 16S-23S rDNA is large, and the variety and quantity of tRNA are distinct. The 16S-23S rDNA intergenic spacer region can be used as an effective supplement of 16S rRNA sequence phylogenetic analysis. Terminal products of the strains detected by gas chromatography are similar and exhibit close phylogenetic relationship. It can be seen as the foundation of identification, and the sequence of 16S rDNA is the main foundation of classification. For stains in dispute, the phylogenetic analysis on them should be according to the morphological, physiological and biochemical characteristics, 16S-23S rDNA intergenic spacer region sequence, and the analysis of terminal products by gas chromatography.
| Original language | English |
|---|---|
| Pages (from-to) | 1965-1968 |
| Number of pages | 4 |
| Journal | Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology |
| Volume | 40 |
| Issue number | 12 |
| State | Published - Dec 2008 |
Keywords
- 16S rDNA
- 16S-23S rDNA intergenic spacer region
- Phylogenetic analysis
- Sulfate reducing bacteria
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