Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2 Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation

Jinming Li; Chris Wai Tung Leung; Dexter Siu Hong Wong; Jianbin Xu; Rui Li; Yueyue Zhao; Chris Yu Yee Yung; Engui Zhao; Ben Zhong Tang; Liming Bian

doi:10.1021/acsami.7b00845

Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF₄:Yb³⁺Tm³⁺@SiO₂ Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation

Jinming Li
, Chris Wai Tung Leung
, Dexter Siu Hong Wong
, Jianbin Xu
, Rui Li
, Yueyue Zhao
, Chris Yu Yee Yung
, Engui Zhao
, Ben Zhong Tang^*
, Liming Bian

^*Corresponding author for this work

Chinese University of Hong Kong
Hong Kong University of Science and Technology
China Orthopedic Regenerative Medicine Group (CORMed)

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).

Original language	English
Pages (from-to)	22074-22084
Number of pages	11
Journal	ACS Applied Materials and Interfaces
Volume	11
Issue number	25
DOIs	https://doi.org/10.1021/acsami.7b00845
State	Published - 26 Jun 2019
Externally published	Yes

Keywords

aggregation-induced emission
controlled differentiation
real-time monitoring cell differentiation
stem cell
up-conversion nanoparticles

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10.1021/acsami.7b00845

Cite this

Li, J., Leung, C. W. T., Wong, D. S. H., Xu, J., Li, R., Zhao, Y., Yung, C. Y. Y., Zhao, E., Tang, B. Z., & Bian, L. (2019). Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF₄:Yb³⁺Tm³⁺@SiO₂ Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation. ACS Applied Materials and Interfaces, 11(25), 22074-22084. https://doi.org/10.1021/acsami.7b00845

@article{cd29b605df074486987e5e80e07d9648,

title = "Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2 Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation",

abstract = "Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).",

keywords = "aggregation-induced emission, controlled differentiation, real-time monitoring cell differentiation, stem cell, up-conversion nanoparticles",

author = "Jinming Li and Leung, \{Chris Wai Tung\} and Wong, \{Dexter Siu Hong\} and Jianbin Xu and Rui Li and Yueyue Zhao and Yung, \{Chris Yu Yee\} and Engui Zhao and Tang, \{Ben Zhong\} and Liming Bian",

note = "Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2019",

month = jun,

day = "26",

doi = "10.1021/acsami.7b00845",

language = "英语",

volume = "11",

pages = "22074--22084",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

number = "25",

}

Li, J, Leung, CWT, Wong, DSH, Xu, J, Li, R, Zhao, Y, Yung, CYY, Zhao, E, Tang, BZ & Bian, L 2019, 'Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF₄:Yb³⁺Tm³⁺@SiO₂ Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation', ACS Applied Materials and Interfaces, vol. 11, no. 25, pp. 22074-22084. https://doi.org/10.1021/acsami.7b00845

Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF₄:Yb³⁺Tm³⁺@SiO₂ Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation. / Li, Jinming; Leung, Chris Wai Tung; Wong, Dexter Siu Hong et al.
In: ACS Applied Materials and Interfaces, Vol. 11, No. 25, 26.06.2019, p. 22074-22084.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF4:Yb3+Tm3+@SiO2 Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation

AU - Li, Jinming

AU - Leung, Chris Wai Tung

AU - Wong, Dexter Siu Hong

AU - Xu, Jianbin

AU - Li, Rui

AU - Zhao, Yueyue

AU - Yung, Chris Yu Yee

AU - Zhao, Engui

AU - Tang, Ben Zhong

AU - Bian, Liming

PY - 2019/6/26

Y1 - 2019/6/26

N2 - Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).

AB - Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).

KW - aggregation-induced emission

KW - controlled differentiation

KW - real-time monitoring cell differentiation

KW - stem cell

KW - up-conversion nanoparticles

UR - https://www.scopus.com/pages/publications/85068107600

U2 - 10.1021/acsami.7b00845

DO - 10.1021/acsami.7b00845

M3 - 文章

C2 - 28350958

AN - SCOPUS:85068107600

SN - 1944-8244

VL - 11

SP - 22074

EP - 22084

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 25

ER -

Photocontrolled SiRNA Delivery and Biomarker-Triggered Luminogens of Aggregation-Induced Emission by Up-Conversion NaYF₄:Yb³⁺Tm³⁺@SiO₂ Nanoparticles for Inducing and Monitoring Stem-Cell Differentiation

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Keywords

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