Performance and microbial community of simultaneous removal of NO₃ ⁻-N, Cd²⁺ and Ca²⁺ in MBBR

A moving-bed biofilm reactor (MBBR) containing immobilized Acinetobacter sp.CN86 was operated to investigate the simultaneous denitrification, bio-mineralization and cadmium removal performance. Effects of hydraulic residence time (HRT) (4 h, 6 h and 8 h), pH (6.0, 7.0 and 8.0) and influent Cd²⁺ concentrations (10 mg/L, 30 mg/L and 50 mg/L) were assessed on the simultaneous removal of nitrate, Cd²⁺ and Ca²⁺. Results indicate that the highest pollutant removal efficiency (98.33% (1.866 mg/L·h) for NO₃ ⁻-N; 99.36% (1.242 mg/L·h) for Cd²⁺; 68.80% (15.480 mg/L·h) for Ca²⁺) was achieved under the conditions of a hydraulic residence time of 8 h, pH of 7.0 and initial Cd²⁺ concentration of 10 mg/L. Analyses of microbial distribution and community structures showed that Acinetobacter sp.CN86 was the main contributor (occupy 15.3% at the species level) to the effective removal of multiple pollutants in the MBBR. In addition, the main gas and precipitation components in the biofilm reactor were identified by gas chromatography, scanning electron microscope, and X-ray diffraction analyses.