Partial nitrification of wastewaters with high NaCl concentrations by aerobic granules in continuous-flow reactor

Wastewaters with high salinity are yielded that need sufficient treatment. This study applied aerobic granules to conduct partial nitrification reactions for wastewaters with high NaCl concentrations in a continuous-flow reactor. The present granules revealed partial nitrification performances at nitrite accumulation rate >95% and chemical oxygen demand (COD) removal at >85% at salt concentration up to 50g l^-1. High salinity led to compact and tough granules. The granules applied electrogenic ion pump and sodium-calcium exchanger to reduce intracellular Na⁺ concentration; generated amino acids as osmoprotectants to resist the high osmotic pressure; produced excess extracellular polysaccharides and proteins with secretion of c-di-GMP; revised microbial community with halophilic strains. The present continuous-flow aerobic granule reactor (CFAGR) is a promising process to convert ammonium in highly saline wastewaters to nitrite, which can be applied with a subsequent Anammox process for efficient nitrogen removal.