Optical measuring methods and apparatus for hemoglobin concentration in whole blood

A portable and accurate optical measuring system for hemoglobin is developed, and its measuring principle, concentration equation, medicament micro-tablet, measurement apparatus and control architecture are also studied. Firstly, the secondary absorb measurement mechanism is designed based on the absorbing spectrum of azide methemoglobin, and the concentration equation is derived. Then the micro-tablet for optical measuring is designed, in which the medicament micro-tablet is hydrophilic to insure the whole blood capillary absorption and to achieve the accuracy and consistency of optical measuring. Finally, the measuring mechanism and control system are introduced in detail, which demonstrates that the measuring system can detect the hemoglobin concentration within 30-60s. Moreover, the clinical trials show that the correlation of the measurement results between the proposed method and the cyaniding methemoglobin (Fe³⁺) method is up to 0.9836 and the veracity can be 96.32%. Because of the portability, no contamination and fast test on line, the instrument can be widely used in moving blood collecting, function judging of the athletes and family health cares.