Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration: Efficiency, iron-compounds transformation, N2O and NO2                             − accumulation, and microbial characteristics

Shihai Deng; Desheng Li; Xue Yang; Qinqing Cai; Shuai Peng; Xinnan Peng; Hong Yao; Binghan Xie

doi:10.1016/j.cej.2019.123409

Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration: Efficiency, iron-compounds transformation, N₂O and NO₂ ⁻ accumulation, and microbial characteristics

Shihai Deng
, Desheng Li^*
, Xue Yang
, Qinqing Cai
, Shuai Peng
, Xinnan Peng
, Hong Yao
, Binghan Xie

^*Corresponding author for this work

Beijing Jiaotong University
National University of Singapore
National Institute of Clean-and-Low-Carbon Energy
School of Marine Science and Technology, Harbin Institute of Technology Weihai

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

This study revealed novel characteristics of the Fe(0)-oxidizing autotrophic denitrification [Fe(0)-ADN] process with aeration, including nitrogen removal efficiency, electron donor transformation, N₂O/NO₂ ⁻ accumulation, and variation of microbial communities and functional genes/enzymes. Iron-scraps (ISs) was utilized as Fe(0) source under the mediation of micro-electrolysis for ISs-ADN process establishment. The anaerobic ISs-ADN process achieved an initial denitrification load of 0.12 ± 0.02 kgN/(m³·d), and it subsequently shifted to 0.34 ± 0.01 kgN/(m³·d) when dissolved oxygen (DO) was increased from 0.1 ± 0.1 mg/L to 1.6 ± 0.1 mg/L. Significant accumulations of N₂O (0.9 ± 0.2 mgN₂O-N/L) and NO₂ ⁻ (5.5 ± 0.7 mgNO₂ ⁻-N/L) were observed in the anaerobic ISs-ADN system, they were reduced to 0.07 ± 0.02 mgN/L and 0.2 ± 0.2 mgN/L, respectively, at DO of 1.6 ± 0.1 mg/L. X-ray diffraction spectra showed that oxygen could enhance bioconversion of Fe₃O₄ into FeO(OH) and reduce the Fe(0)-coating by Fe₃O₄, and hence enhance the ME process for H₂/Fe²⁺ production. High-throughput sequencing results showed that iron-compound transformation bacteria (e.g. Sphaerotillus) and facultative autotrophic denitrificans (e.g. Hydrogenophaga) were enriched at higher DO of 1.6 ± 0.1 mg/L. Genes napA (encoding NAP), nirK (encoding NIR) and nosZ (encoding NosZ) exhibited higher oxygen tolerance. Enzymes NAP, NIR and NosZ maintained the aerobic denitrification, and NIR and NosZ inhibited the accumulation of N₂O and NO₂ ⁻, respectively. Exorbitant aeration (DO ≥ 2.2 ± 0.2 mg/L) accelerated iron oxidation to Fe₃O₄ on the ISs surface and restrained ISs-ADN. This study provides an in-depth understanding on the impact of oxygen on the Fe(0)-ADN. The micro-electrolysis mediated ISs-ADN is competitive in both efficiency and cost with the utilization of ISs.

Original language	English
Article number	123409
Journal	Chemical Engineering Journal
Volume	387
DOIs	https://doi.org/10.1016/j.cej.2019.123409
State	Published - 1 May 2020
Externally published	Yes

Keywords

Fe(0)-oxidizing autotrophic denitrification [Fe(0)-ADN]
Iron scraps
Micro-electrolysis
Microbial characteristics
NO and NO accumulation
Oxygen

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Deng, S., Li, D., Yang, X., Cai, Q., Peng, S., Peng, X., Yao, H., & Xie, B. (2020). Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration: Efficiency, iron-compounds transformation, N₂O and NO₂ ⁻ accumulation, and microbial characteristics. Chemical Engineering Journal, 387, Article 123409. https://doi.org/10.1016/j.cej.2019.123409

@article{ab8657f34fa144a48ad302d08ed9997c,

title = "Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration: Efficiency, iron-compounds transformation, N2O and NO2 − accumulation, and microbial characteristics",

abstract = "This study revealed novel characteristics of the Fe(0)-oxidizing autotrophic denitrification [Fe(0)-ADN] process with aeration, including nitrogen removal efficiency, electron donor transformation, N2O/NO2 − accumulation, and variation of microbial communities and functional genes/enzymes. Iron-scraps (ISs) was utilized as Fe(0) source under the mediation of micro-electrolysis for ISs-ADN process establishment. The anaerobic ISs-ADN process achieved an initial denitrification load of 0.12 ± 0.02 kgN/(m3·d), and it subsequently shifted to 0.34 ± 0.01 kgN/(m3·d) when dissolved oxygen (DO) was increased from 0.1 ± 0.1 mg/L to 1.6 ± 0.1 mg/L. Significant accumulations of N2O (0.9 ± 0.2 mgN2O-N/L) and NO2 − (5.5 ± 0.7 mgNO2 −-N/L) were observed in the anaerobic ISs-ADN system, they were reduced to 0.07 ± 0.02 mgN/L and 0.2 ± 0.2 mgN/L, respectively, at DO of 1.6 ± 0.1 mg/L. X-ray diffraction spectra showed that oxygen could enhance bioconversion of Fe3O4 into FeO(OH) and reduce the Fe(0)-coating by Fe3O4, and hence enhance the ME process for H2/Fe2+ production. High-throughput sequencing results showed that iron-compound transformation bacteria (e.g. Sphaerotillus) and facultative autotrophic denitrificans (e.g. Hydrogenophaga) were enriched at higher DO of 1.6 ± 0.1 mg/L. Genes napA (encoding NAP), nirK (encoding NIR) and nosZ (encoding NosZ) exhibited higher oxygen tolerance. Enzymes NAP, NIR and NosZ maintained the aerobic denitrification, and NIR and NosZ inhibited the accumulation of N2O and NO2 −, respectively. Exorbitant aeration (DO ≥ 2.2 ± 0.2 mg/L) accelerated iron oxidation to Fe3O4 on the ISs surface and restrained ISs-ADN. This study provides an in-depth understanding on the impact of oxygen on the Fe(0)-ADN. The micro-electrolysis mediated ISs-ADN is competitive in both efficiency and cost with the utilization of ISs.",

keywords = "Fe(0)-oxidizing autotrophic denitrification [Fe(0)-ADN], Iron scraps, Micro-electrolysis, Microbial characteristics, NO and NO accumulation, Oxygen",

author = "Shihai Deng and Desheng Li and Xue Yang and Qinqing Cai and Shuai Peng and Xinnan Peng and Hong Yao and Binghan Xie",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2020",

month = may,

day = "1",

doi = "10.1016/j.cej.2019.123409",

language = "英语",

volume = "387",

journal = "Chemical Engineering Journal",

issn = "1385-8947",

publisher = "Elsevier B.V.",

}

Deng, S, Li, D, Yang, X, Cai, Q, Peng, S, Peng, X, Yao, H & Xie, B 2020, 'Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration: Efficiency, iron-compounds transformation, N₂O and NO₂ ⁻ accumulation, and microbial characteristics', Chemical Engineering Journal, vol. 387, 123409. https://doi.org/10.1016/j.cej.2019.123409

Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration: Efficiency, iron-compounds transformation, N₂O and NO₂ ⁻ accumulation, and microbial characteristics. / Deng, Shihai; Li, Desheng; Yang, Xue et al.
In: Chemical Engineering Journal, Vol. 387, 123409, 01.05.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration

T2 - Efficiency, iron-compounds transformation, N2O and NO2 − accumulation, and microbial characteristics

AU - Deng, Shihai

AU - Li, Desheng

AU - Yang, Xue

AU - Cai, Qinqing

AU - Peng, Shuai

AU - Peng, Xinnan

AU - Yao, Hong

AU - Xie, Binghan

PY - 2020/5/1

Y1 - 2020/5/1

N2 - This study revealed novel characteristics of the Fe(0)-oxidizing autotrophic denitrification [Fe(0)-ADN] process with aeration, including nitrogen removal efficiency, electron donor transformation, N2O/NO2 − accumulation, and variation of microbial communities and functional genes/enzymes. Iron-scraps (ISs) was utilized as Fe(0) source under the mediation of micro-electrolysis for ISs-ADN process establishment. The anaerobic ISs-ADN process achieved an initial denitrification load of 0.12 ± 0.02 kgN/(m3·d), and it subsequently shifted to 0.34 ± 0.01 kgN/(m3·d) when dissolved oxygen (DO) was increased from 0.1 ± 0.1 mg/L to 1.6 ± 0.1 mg/L. Significant accumulations of N2O (0.9 ± 0.2 mgN2O-N/L) and NO2 − (5.5 ± 0.7 mgNO2 −-N/L) were observed in the anaerobic ISs-ADN system, they were reduced to 0.07 ± 0.02 mgN/L and 0.2 ± 0.2 mgN/L, respectively, at DO of 1.6 ± 0.1 mg/L. X-ray diffraction spectra showed that oxygen could enhance bioconversion of Fe3O4 into FeO(OH) and reduce the Fe(0)-coating by Fe3O4, and hence enhance the ME process for H2/Fe2+ production. High-throughput sequencing results showed that iron-compound transformation bacteria (e.g. Sphaerotillus) and facultative autotrophic denitrificans (e.g. Hydrogenophaga) were enriched at higher DO of 1.6 ± 0.1 mg/L. Genes napA (encoding NAP), nirK (encoding NIR) and nosZ (encoding NosZ) exhibited higher oxygen tolerance. Enzymes NAP, NIR and NosZ maintained the aerobic denitrification, and NIR and NosZ inhibited the accumulation of N2O and NO2 −, respectively. Exorbitant aeration (DO ≥ 2.2 ± 0.2 mg/L) accelerated iron oxidation to Fe3O4 on the ISs surface and restrained ISs-ADN. This study provides an in-depth understanding on the impact of oxygen on the Fe(0)-ADN. The micro-electrolysis mediated ISs-ADN is competitive in both efficiency and cost with the utilization of ISs.

AB - This study revealed novel characteristics of the Fe(0)-oxidizing autotrophic denitrification [Fe(0)-ADN] process with aeration, including nitrogen removal efficiency, electron donor transformation, N2O/NO2 − accumulation, and variation of microbial communities and functional genes/enzymes. Iron-scraps (ISs) was utilized as Fe(0) source under the mediation of micro-electrolysis for ISs-ADN process establishment. The anaerobic ISs-ADN process achieved an initial denitrification load of 0.12 ± 0.02 kgN/(m3·d), and it subsequently shifted to 0.34 ± 0.01 kgN/(m3·d) when dissolved oxygen (DO) was increased from 0.1 ± 0.1 mg/L to 1.6 ± 0.1 mg/L. Significant accumulations of N2O (0.9 ± 0.2 mgN2O-N/L) and NO2 − (5.5 ± 0.7 mgNO2 −-N/L) were observed in the anaerobic ISs-ADN system, they were reduced to 0.07 ± 0.02 mgN/L and 0.2 ± 0.2 mgN/L, respectively, at DO of 1.6 ± 0.1 mg/L. X-ray diffraction spectra showed that oxygen could enhance bioconversion of Fe3O4 into FeO(OH) and reduce the Fe(0)-coating by Fe3O4, and hence enhance the ME process for H2/Fe2+ production. High-throughput sequencing results showed that iron-compound transformation bacteria (e.g. Sphaerotillus) and facultative autotrophic denitrificans (e.g. Hydrogenophaga) were enriched at higher DO of 1.6 ± 0.1 mg/L. Genes napA (encoding NAP), nirK (encoding NIR) and nosZ (encoding NosZ) exhibited higher oxygen tolerance. Enzymes NAP, NIR and NosZ maintained the aerobic denitrification, and NIR and NosZ inhibited the accumulation of N2O and NO2 −, respectively. Exorbitant aeration (DO ≥ 2.2 ± 0.2 mg/L) accelerated iron oxidation to Fe3O4 on the ISs surface and restrained ISs-ADN. This study provides an in-depth understanding on the impact of oxygen on the Fe(0)-ADN. The micro-electrolysis mediated ISs-ADN is competitive in both efficiency and cost with the utilization of ISs.

KW - Fe(0)-oxidizing autotrophic denitrification [Fe(0)-ADN]

KW - Iron scraps

KW - Micro-electrolysis

KW - Microbial characteristics

KW - NO and NO accumulation

KW - Oxygen

UR - https://www.scopus.com/pages/publications/85076549807

U2 - 10.1016/j.cej.2019.123409

DO - 10.1016/j.cej.2019.123409

M3 - 文章

AN - SCOPUS:85076549807

SN - 1385-8947

VL - 387

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

M1 - 123409

ER -

Deng S, Li D, Yang X, Cai Q, Peng S, Peng X et al. Novel characteristics on micro-electrolysis mediated Fe(0)-oxidizing autotrophic denitrification with aeration: Efficiency, iron-compounds transformation, N₂O and NO₂ ⁻ accumulation, and microbial characteristics. Chemical Engineering Journal. 2020 May 1;387:123409. doi: 10.1016/j.cej.2019.123409