Nitrogen removal performance and microbial characteristics during simultaneous chemical phosphorus removal process using Fe³⁺

To evaluate the effect of Fe³⁺ on nitrogen (N) removal and associated microbial characteristics during simultaneous chemical phosphorus (P) removal, a sequencing batch reactor was used to analyze the changes in the microbial community and metabolic pathways caused by Fe³⁺ addition. Results demonstrated that Fe³⁺ promoted ammonia nitrogen (NH₄⁺-N) removal and inhibited denitrification process, and increased the sludge particles (D50) and the biomass per sludge particle size. Furthermore, the abundances of denitrifying bacteria (Haliangium and Terrimonas) and biological phosphorus removing bacteria (Halaingium, norank_f_Saprospiraceae and SM1A02) were decreased. On the contrary, the increase of nitrifying bacteria abundance and the coding genes of nitrification-related enzymes confirmed the promotion for nitrification with Fe³⁺ addition. Besides, Fe³⁺ inhibited the interspecific relationship between denitrifying bacteria genera and other genera to reduce denitrification efficiency.