Nitrification and denitrification characteristics of new heterotrophic nitrifying bacteria

Two new heterotrophic nitrifying bacteria, wgy5 and wgy33, were isolated from a bio-ceramic reactor. With the aid of sequencing the 16SrDNA and physiological and biochemical identification, they were proved to belong to Pseudomonas sp. and uncultured Acinetobacter sp., respectively. Then the phylogenic tree was established. Sodium acetate and ammonium chloride were used as carbon and nitrogen sources to investigate nitrification characteristics of the two new bacteria. After 12-day aerobic culture, the removal rates of the NH₄-N by wgy5 and wgy33 were 82.86% and 78.30% respectively, and the removal rates of the total concentration of nitrogen were 65.50% and 61.06%. And the two new bacteria have nitrification function of producing NO₂-N. After 12-day aerobic culture, the removal rates of the NO₂-N by wgy5 and wgy33 were 82.20% and 80.36% in nitrosifying culture medium, and the removal rates of the NO₃-N were 84.10% and 90.12% in nitrifying culture medium.