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Nanoparticles for In Vivo Lifetime Multiplexed Imaging

  • Erving Ximendes
  • , Emma Martín Rodríguez
  • , Dirk H. Ortgies
  • , Meiling Tan
  • , Guanying Chen
  • , Blanca del Rosal*
  • *Corresponding author for this work
  • Ramón y Cajal Health Research Institute
  • Universidad Autónoma de Madrid
  • School of Chemistry and Chemical Engineering, Harbin Institute of Technology
  • Royal Melbourne Institute of Technology University

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Lifetime multiplexed imaging refers to the simultaneous labeling of different structures with fluorescent probes that present identical photoluminescence spectra and distinct fluorescence lifetimes. This technique allows extracting quantitative information from multichannel in vivo fluorescence imaging. In vivo lifetime multiplexed imaging requires fluorophores with excitation and emission bands in the near-infrared (NIR) and tunable fluorescence lifetimes, plus an imaging system capable of time-resolved image acquisition and analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages239-251
Number of pages13
DOIs
StatePublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2350
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bioimaging
  • Near-infrared imaging
  • Rare-earth-doped nanoparticles

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