Mutagenesis of three chemical mutagen on hydrogen-producing bacterium ethanoligenens sp.ZGX4

A preliminary study on chemical mutagenesis of diethyl sulfate (DES), 1-methyl-3-nitryl-1-nitrosoguanidine (NTG) and acridine yellow on H₂-producing strain Ethanoligenens sp.ZGX4 was discussed and showed different mutagenic effects. Four kinds of mutants were obtained by DES mutagenesis, among which included a kind of H₂-producing mutant with stronger auto flocculation capacity; The death rate of NTG was maximum and attained one kind of mutant; The death rate of acridine yellow was 21.8%-23.1% and 2 kinds of mutant were got. Compared with NTG and acridine yellow, the mutagenesis of DES was the best. The morphological characteristic of high efficient mutants was similar with that of control strain except that colonies diameter became bigger and transparent.