Multispectral Chiral Quasi-Bound States in the Continuum Enabled Microfluidics for High-Throughput Molecular Screening and Quantification

Traditional chiral detection methods often suffer from label dependence and limited throughput, hindering accurate profiling of trace enantiomers. Herein, a terahertz (THz) multispectral metachip-enabled microfluidic platform is presented for label-free, high-throughput screening and quantitation of chiral molecules. The metachip integrates arrayed pixels engineered with chiral quasi-bound states in the continuum (q-BIC), generating high circular dichroism (CD) across 0.5–2.0 THz. On-chip microfluidic integration enables multi-dimensional CD feature extraction in aqueous environments, while the Uniform Manifold Approximation and Projection (UMAP) algorithm maps multidimensional CD features into a Two-dimensional (2D) spectrum, simultaneously realizing conformation identification and concentration quantification (0.05–0.3 mg dL⁻¹). Experimentally, 87.5% discrimination accuracy is achieved for 8 chiral biomolecules in aqueous solutions (including amino acids and dipeptides) with state-of-the-art sensitivity (2.1024 THz· mg¹ ·dL). This platform bridges the gap between high-sensitivity chiral sensing and real-time fluidic analysis, offering a transformative tool for trace enantiomer characterization in clinical diagnostics and drug development.