Motor protein CF₀F₁ reconstituted in lipid-coated hemoglobin microcapsules for ATP synthesis

A study was conducted to demonstrate the the reconstitution of chloroplastic F₀F₁-ATPase (CF₁F ₀ATPase) within lipid-bilayer-coated hemoglobin (Hb) protein microcapsules. It was also demonstrated that a proton gradient is produced by the hydrolysis of glucose, catalyzed by glucose oxidase (GOD) within the bulk solution outside the capsules. It was found that the proton gradient can be maintained for a longer period of time by the addition of glucose, enabling the ATPase, to be derived from the synthesis of ATP. The study conducted investigations, using a method, which was based on glutaraldehyde (GA), as a covalent crosslinker, to fabricate GA/Hb microcapsules through the LbL assembly technique. The use of GA to crosslink proteins has been shown to lead to significant improvements in the permeability of Hb protein capsules.