Molecular biological methods in environmental engineering

Chunying Li; Fujun Xia; Yuhua Zhang; Chein Chi Chang; Dong Wei; Li Wei

doi:10.2175/106143017X15023776270197

Molecular biological methods in environmental engineering

Chunying Li
, Fujun Xia
, Yuhua Zhang
, Chein Chi Chang
, Dong Wei
, Li Wei

School of Environment

Harbin University of Commerce
Daqing Institute of Oilfield Construction and Design
Hong Kong University of Science and Technology
Sewer Authority
Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Microorganisms can respond fast to the environmental changes, and well suited for environmental assessment. DNA and RNA are generally extracted by extraction kits. After extraction, molecular biological methods including genomics, transcriptomics, proteomics and metabolomics are applied to characterize the genetic and functional diversity of microorganisms. Quantitative PCR, FISH and mass sequence of amplicons are also described. This review provides environmental engineers and microbiologists an overview of important advancements in molecular techniques and highlights the application of these methods in diverse environments.

Original language	English
Pages (from-to)	942-959
Number of pages	18
Journal	Water Environment Research
Volume	89
Issue number	10
DOIs	https://doi.org/10.2175/106143017X15023776270197
State	Published - 2017

Keywords

DNA and RNA extraction
Fluorescent in situ hybridization (FISH)
Microbial molecular techniques
Quantitative PCR (qPCR)

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10.2175/106143017X15023776270197

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title = "Molecular biological methods in environmental engineering",

abstract = "Microorganisms can respond fast to the environmental changes, and well suited for environmental assessment. DNA and RNA are generally extracted by extraction kits. After extraction, molecular biological methods including genomics, transcriptomics, proteomics and metabolomics are applied to characterize the genetic and functional diversity of microorganisms. Quantitative PCR, FISH and mass sequence of amplicons are also described. This review provides environmental engineers and microbiologists an overview of important advancements in molecular techniques and highlights the application of these methods in diverse environments.",

keywords = "DNA and RNA extraction, Fluorescent in situ hybridization (FISH), Microbial molecular techniques, Quantitative PCR (qPCR)",

author = "Chunying Li and Fujun Xia and Yuhua Zhang and Chang, \{Chein Chi\} and Dong Wei and Li Wei",

year = "2017",

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journal = "Water Environment Research",

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T1 - Molecular biological methods in environmental engineering

AU - Li, Chunying

AU - Xia, Fujun

AU - Zhang, Yuhua

AU - Chang, Chein Chi

AU - Wei, Dong

AU - Wei, Li

PY - 2017

Y1 - 2017

N2 - Microorganisms can respond fast to the environmental changes, and well suited for environmental assessment. DNA and RNA are generally extracted by extraction kits. After extraction, molecular biological methods including genomics, transcriptomics, proteomics and metabolomics are applied to characterize the genetic and functional diversity of microorganisms. Quantitative PCR, FISH and mass sequence of amplicons are also described. This review provides environmental engineers and microbiologists an overview of important advancements in molecular techniques and highlights the application of these methods in diverse environments.

AB - Microorganisms can respond fast to the environmental changes, and well suited for environmental assessment. DNA and RNA are generally extracted by extraction kits. After extraction, molecular biological methods including genomics, transcriptomics, proteomics and metabolomics are applied to characterize the genetic and functional diversity of microorganisms. Quantitative PCR, FISH and mass sequence of amplicons are also described. This review provides environmental engineers and microbiologists an overview of important advancements in molecular techniques and highlights the application of these methods in diverse environments.

KW - DNA and RNA extraction

KW - Fluorescent in situ hybridization (FISH)

KW - Microbial molecular techniques

KW - Quantitative PCR (qPCR)

UR - https://www.scopus.com/pages/publications/85033217542

U2 - 10.2175/106143017X15023776270197

DO - 10.2175/106143017X15023776270197

M3 - 文章

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SN - 1061-4303

VL - 89

SP - 942

EP - 959

JO - Water Environment Research

JF - Water Environment Research

IS - 10

ER -

Molecular biological methods in environmental engineering

Abstract

Keywords

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