Microfluidics-Prepared Uniform Conjugated Polymer Nanoparticles for Photo-Triggered Immune Microenvironment Modulation and Cancer Therapy

Photothermal therapy (PTT) has shown great promise to spatiotemporally ablate cancer cells, and further understanding of the immune system response to PTT treatment would contribute to improvement in therapeutic outcomes. Herein, we utilize microfluidic technology to prepare biocompatible conjugated polymer nanoparticles (CP NPs) as PTT agents and assess the immune response triggered by CP-based PTT treatment in vitro and in vivo. Through careful control of the antisolvent, CP NPs with a uniform diameter of 52 nm were obtained. The c-RGD-functionalized CP NPs exhibit high photothermal conversion efficiency, inducing effective cancer cell death under an 808 nm laser illumination. Using macrophage cells as the model, CP NPs demonstrate effective activation of proinflammatory immune response. Furthermore, in tumor-bearing mice model, a single round of CP NP-assisted PTT could efficiently induce antitumor immunity activation and ultimately inhibit tumor growth. The study provides detailed understanding of both microfluidic technology for CP NP fabrication and photothermal-triggered antitumor immune responses.