Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry

Xiaojun Han; Cees Van Berkel; James Gwyer; Lorenzo Capretto; Hywel Morgan

doi:10.1021/ac202700x

Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry

Xiaojun Han
, Cees Van Berkel
, James Gwyer
, Lorenzo Capretto
, Hywel Morgan^*

^*Corresponding author for this work

University of Southampton
Koninklijke Philips N.V.

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using experimental and simulated results. Healthy and clinical bloods analyzed with this system, and the data shows good correlation against data obtained from commercial hematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R ² (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes, and 0.99 for granulocytes in terms of relative counts and 0.94 for lymphocytes, 0.91 for monocytes, and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose.

Original language	English
Pages (from-to)	1070-1075
Number of pages	6
Journal	Analytical Chemistry
Volume	84
Issue number	2
DOIs	https://doi.org/10.1021/ac202700x
State	Published - 17 Jan 2012
Externally published	Yes

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title = "Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry",

abstract = "This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using experimental and simulated results. Healthy and clinical bloods analyzed with this system, and the data shows good correlation against data obtained from commercial hematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R 2 (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes, and 0.99 for granulocytes in terms of relative counts and 0.94 for lymphocytes, 0.91 for monocytes, and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose.",

author = "Xiaojun Han and \{Van Berkel\}, Cees and James Gwyer and Lorenzo Capretto and Hywel Morgan",

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doi = "10.1021/ac202700x",

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T1 - Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry

AU - Han, Xiaojun

AU - Van Berkel, Cees

AU - Gwyer, James

AU - Capretto, Lorenzo

AU - Morgan, Hywel

PY - 2012/1/17

Y1 - 2012/1/17

N2 - This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using experimental and simulated results. Healthy and clinical bloods analyzed with this system, and the data shows good correlation against data obtained from commercial hematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R 2 (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes, and 0.99 for granulocytes in terms of relative counts and 0.94 for lymphocytes, 0.91 for monocytes, and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose.

AB - This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using experimental and simulated results. Healthy and clinical bloods analyzed with this system, and the data shows good correlation against data obtained from commercial hematology machines. The data from the microfluidic system was compared against hospital data for 18 clinical samples, giving R 2 (coefficient of determination) values of 0.99 for lymphocytes, 0.89 for monocytes, and 0.99 for granulocytes in terms of relative counts and 0.94 for lymphocytes, 0.91 for monocytes, and 0.95 for granulocytes in terms of absolute counts. This demonstrates the potential clinical utility of this new system for a point-of-care purpose.

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U2 - 10.1021/ac202700x

DO - 10.1021/ac202700x

M3 - 文章

C2 - 22148390

AN - SCOPUS:84855930061

SN - 0003-2700

VL - 84

SP - 1070

EP - 1075

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 2

ER -

Microfluidic lysis of human blood for leukocyte analysis using single cell impedance cytometry

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