Method for screening of saccharide-accumulation microfloria from lignocelluloses and saccharification assessment

In order to research the saccharification process of straw and screen efficient saccharification microflora, especially to solve the problem about the capability assessment of cellulose saccharification, a mixed strain was selected by serial dilution method. A new method is proposed, in which the mixed strain and high efficient H₂-producing strain are put in different chambers for hydrogen production culture with simultaneous saccharification and fermentation to evaluate the capability of cellulose saccharification by the amount of glucose consumed for producing hydrogen. The results show that the use of serial dilution switching methods can screen a high efficient saccharification microflora JY665, and the bacterial hydrogen production volume and the amount of reducing sugars can be fitted as a regression curve. By calculating, the maximum reducing sugar yield can reach 0.5150 g/g-straw for the complex strains JY, and the straw degradation rate reaches 61.06%.