Lysozyme imprinted Fe3O4@SiO2 nanoparticles via SI-ATRP with temperature-controlled reversible adsorption

Jun Tian; Yi Pang; Hongjuan Gu; Dongyan Tang; Zaiqian Yu

doi:10.1039/d3nj01345f

Lysozyme imprinted Fe₃O₄@SiO₂ nanoparticles via SI-ATRP with temperature-controlled reversible adsorption

Jun Tian
, Yi Pang
, Hongjuan Gu
, Dongyan Tang
, Zaiqian Yu^*

^*Corresponding author for this work

Changchun University of Technology
Ltd.
School of Chemistry and Chemical Engineering, Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Nanoscale molecularly imprinted polymers (MIPs) could offer high binding capacity and fast mass transfer, but their removal after adsorption is costly and time-consuming. In this paper, Fe₃O₄@SiO₂ nanoparticles were synthesized and a lysozyme-imprinted polymer was synthesized via surface initiated atom transfer polymerization on the surface of Fe₃O₄@SiO₂. Temperature-controlled adsorption and desorption of lysozyme were measured, and the adsorption kinetics and isothermal adsorption were determined. The results indicate that the synthesized Fe₃O₄ has an inverse spinel crystalline structure and Fe₃O₄@SiO₂ is a core-shell structured nanoparticle. The adsorption kinetics followed the Langmuir EXT1 model, and the equilibrium adsorption capacity of the Fe₃O₄@SiO₂@MIP at 40 °C was 117.12 mg g⁻¹ with an imprinting factor of 1.51. SDS-PAGE electrophoresis confirmed the specific adsorption of lysozyme, and 86% of the adsorbed lysozyme could be released by changing the temperature.

Original language	English
Pages (from-to)	9905-9912
Number of pages	8
Journal	New Journal of Chemistry
Volume	47
Issue number	20
DOIs	https://doi.org/10.1039/d3nj01345f
State	Published - 25 Apr 2023
Externally published	Yes

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title = "Lysozyme imprinted Fe3O4@SiO2 nanoparticles via SI-ATRP with temperature-controlled reversible adsorption",

abstract = "Nanoscale molecularly imprinted polymers (MIPs) could offer high binding capacity and fast mass transfer, but their removal after adsorption is costly and time-consuming. In this paper, Fe3O4@SiO2 nanoparticles were synthesized and a lysozyme-imprinted polymer was synthesized via surface initiated atom transfer polymerization on the surface of Fe3O4@SiO2. Temperature-controlled adsorption and desorption of lysozyme were measured, and the adsorption kinetics and isothermal adsorption were determined. The results indicate that the synthesized Fe3O4 has an inverse spinel crystalline structure and Fe3O4@SiO2 is a core-shell structured nanoparticle. The adsorption kinetics followed the Langmuir EXT1 model, and the equilibrium adsorption capacity of the Fe3O4@SiO2@MIP at 40 °C was 117.12 mg g−1 with an imprinting factor of 1.51. SDS-PAGE electrophoresis confirmed the specific adsorption of lysozyme, and 86\% of the adsorbed lysozyme could be released by changing the temperature.",

author = "Jun Tian and Yi Pang and Hongjuan Gu and Dongyan Tang and Zaiqian Yu",

note = "Publisher Copyright: {\textcopyright} 2023 The Royal Society of Chemistry.",

year = "2023",

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day = "25",

doi = "10.1039/d3nj01345f",

language = "英语",

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pages = "9905--9912",

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T1 - Lysozyme imprinted Fe3O4@SiO2 nanoparticles via SI-ATRP with temperature-controlled reversible adsorption

AU - Tian, Jun

AU - Pang, Yi

AU - Gu, Hongjuan

AU - Tang, Dongyan

AU - Yu, Zaiqian

PY - 2023/4/25

Y1 - 2023/4/25

N2 - Nanoscale molecularly imprinted polymers (MIPs) could offer high binding capacity and fast mass transfer, but their removal after adsorption is costly and time-consuming. In this paper, Fe3O4@SiO2 nanoparticles were synthesized and a lysozyme-imprinted polymer was synthesized via surface initiated atom transfer polymerization on the surface of Fe3O4@SiO2. Temperature-controlled adsorption and desorption of lysozyme were measured, and the adsorption kinetics and isothermal adsorption were determined. The results indicate that the synthesized Fe3O4 has an inverse spinel crystalline structure and Fe3O4@SiO2 is a core-shell structured nanoparticle. The adsorption kinetics followed the Langmuir EXT1 model, and the equilibrium adsorption capacity of the Fe3O4@SiO2@MIP at 40 °C was 117.12 mg g−1 with an imprinting factor of 1.51. SDS-PAGE electrophoresis confirmed the specific adsorption of lysozyme, and 86% of the adsorbed lysozyme could be released by changing the temperature.

AB - Nanoscale molecularly imprinted polymers (MIPs) could offer high binding capacity and fast mass transfer, but their removal after adsorption is costly and time-consuming. In this paper, Fe3O4@SiO2 nanoparticles were synthesized and a lysozyme-imprinted polymer was synthesized via surface initiated atom transfer polymerization on the surface of Fe3O4@SiO2. Temperature-controlled adsorption and desorption of lysozyme were measured, and the adsorption kinetics and isothermal adsorption were determined. The results indicate that the synthesized Fe3O4 has an inverse spinel crystalline structure and Fe3O4@SiO2 is a core-shell structured nanoparticle. The adsorption kinetics followed the Langmuir EXT1 model, and the equilibrium adsorption capacity of the Fe3O4@SiO2@MIP at 40 °C was 117.12 mg g−1 with an imprinting factor of 1.51. SDS-PAGE electrophoresis confirmed the specific adsorption of lysozyme, and 86% of the adsorbed lysozyme could be released by changing the temperature.

UR - https://www.scopus.com/pages/publications/85159380340

U2 - 10.1039/d3nj01345f

DO - 10.1039/d3nj01345f

M3 - 文章

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SN - 1144-0546

VL - 47

SP - 9905

EP - 9912

JO - New Journal of Chemistry

JF - New Journal of Chemistry

IS - 20

ER -

Lysozyme imprinted Fe₃O₄@SiO₂ nanoparticles via SI-ATRP with temperature-controlled reversible adsorption

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