Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models

Kyall J. Pocock; Xiaofang Gao; Chenxi Wang; Craig Priest; Clive A. Prestidge; Kazuma Mawatari; Takehiko Kitamori; Benjamin Thierry

doi:10.1117/12.2202461

Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models

Kyall J. Pocock
, Xiaofang Gao
, Chenxi Wang
, Craig Priest
, Clive A. Prestidge
, Kazuma Mawatari
, Takehiko Kitamori
, Benjamin Thierry

School of Materials Science and Engineering

University of South Australia
The University of Tokyo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

The integration of microfluidics with living biological systems has paved the way to the exciting concept of 'organson-a-chip', which aims at the development of advanced in vitro models that replicate the key features of human organs. Glass based devices have long been utilised in the field of microfluidics but the integration of alternative functional elements within multi-layered glass microdevices, such as polymeric membranes, remains a challenge. To this end, we have extended a previously reported approach for the low-temperature bonding of glass devices that enables the integration of a functional polycarbonate porous membrane. The process was initially developed and optimised on specialty low-temperature bonding equipment (μTAS2001, Bondtech, Japan) and subsequently adapted to more widely accessible hot embosser units (EVG520HE Hot Embosser, EVG, Austria). The key aspect of this method is the use of low temperatures compatible with polymeric membranes. Compared to borosilicate glass bonding (650 °C) and quartz/fused silica bonding (1050 °C) processes, this method maintains the integrity and functionality of the membrane (T_g 150 °C for polycarbonate). Leak tests performed showed no damage or loss of integrity of the membrane for up to 150 hours, indicating sufficient bond strength for long term cell culture. A feasibility study confirmed the growth of dense and functional monolayers of Caco-2 cells within 5 days.

Original language	English
Title of host publication	Micro+Nano Materials, Devices, and Systems
Editors	Stefano Palomba, Benjamin J. Eggleton
Publisher	SPIE
ISBN (Electronic)	9781628418903
DOIs	https://doi.org/10.1117/12.2202461
State	Published - 2015
Event	SPIE Micro+Nano Materials, Devices, and Applications Symposium - Sydney, Australia Duration: 6 Dec 2015 → 9 Dec 2015

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	9668
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	SPIE Micro+Nano Materials, Devices, and Applications Symposium
Country/Territory	Australia
City	Sydney
Period	6/12/15 → 9/12/15

Keywords

cell co-culture
intestine-on-a-chip
low temperature bonding
microfluidics
organ-on-a-chip

Access to Document

10.1117/12.2202461

Cite this

Pocock, K. J., Gao, X., Wang, C., Priest, C., Prestidge, C. A., Mawatari, K., Kitamori, T., & Thierry, B. (2015). Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models. In S. Palomba, & B. J. Eggleton (Eds.), Micro+Nano Materials, Devices, and Systems Article 96680W (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9668). SPIE. https://doi.org/10.1117/12.2202461

@inproceedings{e548b328e9274fc6b5be8bceb45f12f2,

title = "Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models",

abstract = "The integration of microfluidics with living biological systems has paved the way to the exciting concept of 'organson-a-chip', which aims at the development of advanced in vitro models that replicate the key features of human organs. Glass based devices have long been utilised in the field of microfluidics but the integration of alternative functional elements within multi-layered glass microdevices, such as polymeric membranes, remains a challenge. To this end, we have extended a previously reported approach for the low-temperature bonding of glass devices that enables the integration of a functional polycarbonate porous membrane. The process was initially developed and optimised on specialty low-temperature bonding equipment (μTAS2001, Bondtech, Japan) and subsequently adapted to more widely accessible hot embosser units (EVG520HE Hot Embosser, EVG, Austria). The key aspect of this method is the use of low temperatures compatible with polymeric membranes. Compared to borosilicate glass bonding (650 °C) and quartz/fused silica bonding (1050 °C) processes, this method maintains the integrity and functionality of the membrane (Tg 150 °C for polycarbonate). Leak tests performed showed no damage or loss of integrity of the membrane for up to 150 hours, indicating sufficient bond strength for long term cell culture. A feasibility study confirmed the growth of dense and functional monolayers of Caco-2 cells within 5 days.",

keywords = "cell co-culture, intestine-on-a-chip, low temperature bonding, microfluidics, organ-on-a-chip",

author = "Pocock, \{Kyall J.\} and Xiaofang Gao and Chenxi Wang and Craig Priest and Prestidge, \{Clive A.\} and Kazuma Mawatari and Takehiko Kitamori and Benjamin Thierry",

note = "Publisher Copyright: {\textcopyright} 2015 SPIE.; SPIE Micro+Nano Materials, Devices, and Applications Symposium ; Conference date: 06-12-2015 Through 09-12-2015",

year = "2015",

doi = "10.1117/12.2202461",

language = "英语",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Stefano Palomba and Eggleton, \{Benjamin J.\}",

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Pocock, KJ, Gao, X, Wang, C, Priest, C, Prestidge, CA, Mawatari, K, Kitamori, T & Thierry, B 2015, Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models. in S Palomba & BJ Eggleton (eds), Micro+Nano Materials, Devices, and Systems., 96680W, Proceedings of SPIE - The International Society for Optical Engineering, vol. 9668, SPIE, SPIE Micro+Nano Materials, Devices, and Applications Symposium, Sydney, Australia, 6/12/15. https://doi.org/10.1117/12.2202461

Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models. / Pocock, Kyall J.; Gao, Xiaofang; Wang, Chenxi et al.
Micro+Nano Materials, Devices, and Systems. ed. / Stefano Palomba; Benjamin J. Eggleton. SPIE, 2015. 96680W (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9668).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models

AU - Pocock, Kyall J.

AU - Gao, Xiaofang

AU - Wang, Chenxi

AU - Priest, Craig

AU - Prestidge, Clive A.

AU - Mawatari, Kazuma

AU - Kitamori, Takehiko

AU - Thierry, Benjamin

PY - 2015

Y1 - 2015

N2 - The integration of microfluidics with living biological systems has paved the way to the exciting concept of 'organson-a-chip', which aims at the development of advanced in vitro models that replicate the key features of human organs. Glass based devices have long been utilised in the field of microfluidics but the integration of alternative functional elements within multi-layered glass microdevices, such as polymeric membranes, remains a challenge. To this end, we have extended a previously reported approach for the low-temperature bonding of glass devices that enables the integration of a functional polycarbonate porous membrane. The process was initially developed and optimised on specialty low-temperature bonding equipment (μTAS2001, Bondtech, Japan) and subsequently adapted to more widely accessible hot embosser units (EVG520HE Hot Embosser, EVG, Austria). The key aspect of this method is the use of low temperatures compatible with polymeric membranes. Compared to borosilicate glass bonding (650 °C) and quartz/fused silica bonding (1050 °C) processes, this method maintains the integrity and functionality of the membrane (Tg 150 °C for polycarbonate). Leak tests performed showed no damage or loss of integrity of the membrane for up to 150 hours, indicating sufficient bond strength for long term cell culture. A feasibility study confirmed the growth of dense and functional monolayers of Caco-2 cells within 5 days.

AB - The integration of microfluidics with living biological systems has paved the way to the exciting concept of 'organson-a-chip', which aims at the development of advanced in vitro models that replicate the key features of human organs. Glass based devices have long been utilised in the field of microfluidics but the integration of alternative functional elements within multi-layered glass microdevices, such as polymeric membranes, remains a challenge. To this end, we have extended a previously reported approach for the low-temperature bonding of glass devices that enables the integration of a functional polycarbonate porous membrane. The process was initially developed and optimised on specialty low-temperature bonding equipment (μTAS2001, Bondtech, Japan) and subsequently adapted to more widely accessible hot embosser units (EVG520HE Hot Embosser, EVG, Austria). The key aspect of this method is the use of low temperatures compatible with polymeric membranes. Compared to borosilicate glass bonding (650 °C) and quartz/fused silica bonding (1050 °C) processes, this method maintains the integrity and functionality of the membrane (Tg 150 °C for polycarbonate). Leak tests performed showed no damage or loss of integrity of the membrane for up to 150 hours, indicating sufficient bond strength for long term cell culture. A feasibility study confirmed the growth of dense and functional monolayers of Caco-2 cells within 5 days.

KW - cell co-culture

KW - intestine-on-a-chip

KW - low temperature bonding

KW - microfluidics

KW - organ-on-a-chip

UR - https://www.scopus.com/pages/publications/84959862915

U2 - 10.1117/12.2202461

DO - 10.1117/12.2202461

M3 - 会议稿件

AN - SCOPUS:84959862915

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Micro+Nano Materials, Devices, and Systems

A2 - Palomba, Stefano

A2 - Eggleton, Benjamin J.

PB - SPIE

T2 - SPIE Micro+Nano Materials, Devices, and Applications Symposium

Y2 - 6 December 2015 through 9 December 2015

ER -

Pocock KJ, Gao X, Wang C, Priest C, Prestidge CA, Mawatari K et al. Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models. In Palomba S, Eggleton BJ, editors, Micro+Nano Materials, Devices, and Systems. SPIE. 2015. 96680W. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2202461

Low-temperature bonded glass-membrane microfluidic device for in vitro organ-on-a-chip cell culture models

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Keywords

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