Abstract
In order to obtain high efficiency aniline-degrading bacteria at low temperature, we chose activated sludge from a wastewater treatment plant of a certain chemical plant. Through domestication, separation and purification, we obtained five high efficiency aniline-degrading bacteria at low temperature named A1, A4, A7, A9 and A14. With the aid of sequencing the 16 S rDNA and establishing the phylogenic tree and the physiological biochemical experiment to identify the bacteria, we studied their removal characteristics. The aniline removal rates of Al, A4, A7, A9 and A14 can achieve 87.5%, 82.9%, 100%, 91.5% and 82.8% respectively, when cultured for 84 h. When cultured for 96 h, they all can achieve 100%. The optimum temperature is 15°C. The temperature range for the growth is 5-35°C. The optimum pH is 7.0. Identification shows that. A4 and A9 belong to Delftia. sp, A7 belongs to Acinetobacter. sp, while Al and A14 belong to Pseudomonas. sp.
| Original language | English |
|---|---|
| Pages (from-to) | 1600-1605 |
| Number of pages | 6 |
| Journal | Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology |
| Volume | 40 |
| Issue number | 10 |
| State | Published - Oct 2008 |
Keywords
- Aniline
- Identification
- Low temperature
- Microorganism degrading
- Separation
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