Isolation of a PHA producing strain with butyric acid as the carbon source and its shaking-flask fermentation character

Zhi Qiang Chen; Yun Bei Li; Qin Xue Wen

Isolation of a PHA producing strain with butyric acid as the carbon source and its shaking-flask fermentation character

Zhi Qiang Chen^*
, Yun Bei Li
, Qin Xue Wen

^*Corresponding author for this work

School of Environment

National Engineering Center of Urban Water Resources
Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A bacterial strain, WD-3, with high polyhydroxyalkanoates (PHA) yield was isolated from activated sludge. Its fermentation characters including the effects of C:N ratio, pH, fermentation time on the PHA production were also primary studied. The results showed that the optimal C/N ratio and pH were 35 and 7.0, respectively. PHA accumulation increased rapidly during the stable growth stage. The maximum PHA yield was 3.01 g · L^-1, the strain accumulated PHA up to 45.4% of its cellular dry weight, and the proportion of PHV occupied one-third of the PHA production. Analysis of its 16S rRNA sequence showed that strain WD-3 belongs to Enterobacter.

Original language	English
Pages (from-to)	828-832
Number of pages	5
Journal	Huanjing Kexue/Environmental Science
Volume	31
Issue number	3
State	Published - Mar 2010

Keywords

Butric acid
C:N ratio
Fermentation time
Polyhydroxyalkanoates (PHA)
Strain

Cite this

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title = "Isolation of a PHA producing strain with butyric acid as the carbon source and its shaking-flask fermentation character",

abstract = "A bacterial strain, WD-3, with high polyhydroxyalkanoates (PHA) yield was isolated from activated sludge. Its fermentation characters including the effects of C:N ratio, pH, fermentation time on the PHA production were also primary studied. The results showed that the optimal C/N ratio and pH were 35 and 7.0, respectively. PHA accumulation increased rapidly during the stable growth stage. The maximum PHA yield was 3.01 g · L-1, the strain accumulated PHA up to 45.4\% of its cellular dry weight, and the proportion of PHV occupied one-third of the PHA production. Analysis of its 16S rRNA sequence showed that strain WD-3 belongs to Enterobacter.",

keywords = "Butric acid, C:N ratio, Fermentation time, Polyhydroxyalkanoates (PHA), Strain",

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journal = "Huanjing Kexue/Environmental Science",

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T1 - Isolation of a PHA producing strain with butyric acid as the carbon source and its shaking-flask fermentation character

AU - Chen, Zhi Qiang

AU - Li, Yun Bei

AU - Wen, Qin Xue

PY - 2010/3

Y1 - 2010/3

N2 - A bacterial strain, WD-3, with high polyhydroxyalkanoates (PHA) yield was isolated from activated sludge. Its fermentation characters including the effects of C:N ratio, pH, fermentation time on the PHA production were also primary studied. The results showed that the optimal C/N ratio and pH were 35 and 7.0, respectively. PHA accumulation increased rapidly during the stable growth stage. The maximum PHA yield was 3.01 g · L-1, the strain accumulated PHA up to 45.4% of its cellular dry weight, and the proportion of PHV occupied one-third of the PHA production. Analysis of its 16S rRNA sequence showed that strain WD-3 belongs to Enterobacter.

AB - A bacterial strain, WD-3, with high polyhydroxyalkanoates (PHA) yield was isolated from activated sludge. Its fermentation characters including the effects of C:N ratio, pH, fermentation time on the PHA production were also primary studied. The results showed that the optimal C/N ratio and pH were 35 and 7.0, respectively. PHA accumulation increased rapidly during the stable growth stage. The maximum PHA yield was 3.01 g · L-1, the strain accumulated PHA up to 45.4% of its cellular dry weight, and the proportion of PHV occupied one-third of the PHA production. Analysis of its 16S rRNA sequence showed that strain WD-3 belongs to Enterobacter.

KW - Butric acid

KW - C:N ratio

KW - Fermentation time

KW - Polyhydroxyalkanoates (PHA)

KW - Strain

UR - https://www.scopus.com/pages/publications/77952002698

M3 - 文章

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AN - SCOPUS:77952002698

SN - 0250-3301

VL - 31

SP - 828

EP - 832

JO - Huanjing Kexue/Environmental Science

JF - Huanjing Kexue/Environmental Science

IS - 3

ER -

Isolation of a PHA producing strain with butyric acid as the carbon source and its shaking-flask fermentation character

Abstract

Keywords

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