Isolation and quantitative detection of adhesive sulfate reducing bacteria

Aiming at the corrosion on the inside wall of the pipeline and the pot due to the adhesive sulfate reducing bacteria (A-SRB), the Hungate anaerobic technique was utilized to realize the isolation, 16S rDNA sequence phylogenetic analysis and quantitative detection on the adhesive sulfate reducing bacteria (A-SRB). The study shows that the adhesive sulfate reducing bacteria (A-SRB) are the majority of the SRB in ground system. The strain is mainly distributed in Finmicutes Clostridia, Proteobacteria Gammaproteobacteria and Deltaproteobacteria. And the Clostridium species is the dominant species. The adhesive sulfate reducing bacteria (A-SRB) are a novel species. The quantitative detection of the adhesive sulfate reducing bacteria (A-SRB) was carried out by the detection device installed on the inside wall of the pipeline and the most probable number (MPN) method. And the technique has already been applied to the oilfield production.