Isolation and identification of phenol degradation bacteria with high efficiency applied in immobilized biological technique

The efforts to work on dominant bacteria and remove the phenolic wastewater were carried out in the study. The strains of phenol degradation bacteria with high efficiency were screened from activated sludge of the aeration basin of the present biochemical treatment system in Harbin Gasification Plant by advanced bio-engineering technology. With the aid of sequencing the 16SDNA and establishing the phylogenic tree, Based on screening and acclimating phenol degradation strains, LmPHs fractions of the highly-effective strains of phenol degradation bacteria were amplified. The strains were fastened to biological activated car-bon (IBAC) by biological immobilization technologies. They were already applied in the medium tested e-quipments of processing coal-gas wastewater in Harbin Gasification Plant. 3 strains of phenol degradation bacteria with high efficiency were screened by media from sludge and the phenol removal rates were higher than 99%. When influent COD of IBAC reactor was below 600 mg/L and phenols of IBAC reactor below 75 mg/L, COD and phenol removal rates were higher than 80% and 70%. They were identified physiologically and biochemically. They were respectively Aeromonas, Bacillus and Acinetobacter. So it is sure to happen that the subsequent research will make 3 strains play an important protective role in the environment.