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Isolation and characterization of seven quorum quenching bacteria for biofouling control in MBR

  • Can Zhang
  • , Shijie Zhan
  • , Jian Wang
  • , Zhongjian Liu
  • , Hong You
  • , Yuhong Jia*
  • *Corresponding author for this work
  • School of Marine Science and Technology, Harbin Institute of Technology Weihai
  • COSCO (Weihai) Shipbuilding Technology Co. Lmt

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, interspecies quorum quenching (QQ) by bacteria has been reported as a novel approach in biofouling control in membrane bioreactor (MBR) for wastewater treatment. In this study, seven QQ bacteria, named RO1 to RO7, were isolated from fouling reverse osmosis (RO) membrane and were encapsulated in the microbial filters for biofouling control in MBR. Results showed that all isolates have extracellular QQ activity, and RO1 to RO6 were characterized as heat-stable enzymatic degradation of N-acyl homoserine lactones (AHLs), while RO7 was mainly pH-dependent. Analysis of the 16S rRNA gene indicated that RO7 was a species of Pseudomonas sp., others belonged to the species of Bacillus, Stenotrophomonas maltophilia and Delftia tsuruhatensis, respectively. Then, the microbial filters encapsulated each isolate were applied in a laboratory-scaled MBR and proved the substantial inhibition effect on membrane biofouling except for RO7, indicating that QQ bacteria employing enzymes to degrade AHLs have great potential in biofouling control in MBR.

Original languageEnglish
Pages (from-to)991-1001
Number of pages11
JournalClean Technologies and Environmental Policy
Volume19
Issue number4
DOIs
StatePublished - 2017
Externally publishedYes

Keywords

  • Biofouling
  • Enzymatic degradation
  • Membrane bioreactor (MBR)
  • N-acylhomoserine lactone (AHL)
  • Quorum quenching (QQ)

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