Inulin increases the EPS biosynthesis of Lactobacillus delbrueckii ssp. bulgaricus LDB-C1

Yuxuan Guan; Yanhua Cui; Qian Wang; Xiaojun Qu

doi:10.1007/s10529-023-03365-z

Inulin increases the EPS biosynthesis of Lactobacillus delbrueckii ssp. bulgaricus LDB-C1

Yuxuan Guan
, Yanhua Cui^*
, Qian Wang
, Xiaojun Qu

^*Corresponding author for this work

School of Medicine and Health, Harbin Institute of Technology
Heilongjiang Academy of Sciences

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Objective: Its eps gene cluster, the antioxidant activity and monosaccharide composition of exopolysaccharides, the expression levels of related genes at different fermentations were analyzed for clarifying the exopolysaccharide biosynthesis mechanism of Lactobacillus delbrueckii subsp. bulgaricus LDB-C1. Results: The comparison analysis of eps gene clusters indicated that the gene clusters present diversity and strain specificity. The crude exopolysaccharides from LDB-C1 exhibited a good antioxidant activity. Compared with glucose, fructose, galactose, and fructooligosaccharide, inulin significantly improved the exopolysaccharide biosynthesis. The structures of EPSs were significantly different under different carbohydrate fermentation conditions. Inulin obviously increased the expressions of most EPS biosynthesis related genes at fermentation 4 h. Conclusion: Inulin accelerated the beginning of the exopolysaccharide production in LDB-C1, and the enzymes promoted by inulin was beneficial for the accumulation of exopolysaccharide at the whole fermentation process.

Original language	English
Pages (from-to)	639-654
Number of pages	16
Journal	Biotechnology Letters
Volume	45
Issue number	5-6
DOIs	https://doi.org/10.1007/s10529-023-03365-z
State	Published - Jun 2023
Externally published	Yes

Keywords

Exopolysaccharide
Inulin
Lactobacillus delbrueckii subsp. bulgaricus
Two component system

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10.1007/s10529-023-03365-z

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@article{cdfc1d0660134d3fb6fa580c494cdeca,

title = "Inulin increases the EPS biosynthesis of Lactobacillus delbrueckii ssp. bulgaricus LDB-C1",

abstract = "Objective: Its eps gene cluster, the antioxidant activity and monosaccharide composition of exopolysaccharides, the expression levels of related genes at different fermentations were analyzed for clarifying the exopolysaccharide biosynthesis mechanism of Lactobacillus delbrueckii subsp. bulgaricus LDB-C1. Results: The comparison analysis of eps gene clusters indicated that the gene clusters present diversity and strain specificity. The crude exopolysaccharides from LDB-C1 exhibited a good antioxidant activity. Compared with glucose, fructose, galactose, and fructooligosaccharide, inulin significantly improved the exopolysaccharide biosynthesis. The structures of EPSs were significantly different under different carbohydrate fermentation conditions. Inulin obviously increased the expressions of most EPS biosynthesis related genes at fermentation 4 h. Conclusion: Inulin accelerated the beginning of the exopolysaccharide production in LDB-C1, and the enzymes promoted by inulin was beneficial for the accumulation of exopolysaccharide at the whole fermentation process.",

keywords = "Exopolysaccharide, Inulin, Lactobacillus delbrueckii subsp. bulgaricus, Two component system",

author = "Yuxuan Guan and Yanhua Cui and Qian Wang and Xiaojun Qu",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer Nature B.V.",

year = "2023",

month = jun,

doi = "10.1007/s10529-023-03365-z",

language = "英语",

volume = "45",

pages = "639--654",

journal = "Biotechnology Letters",

issn = "0141-5492",

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TY - JOUR

T1 - Inulin increases the EPS biosynthesis of Lactobacillus delbrueckii ssp. bulgaricus LDB-C1

AU - Guan, Yuxuan

AU - Cui, Yanhua

AU - Wang, Qian

AU - Qu, Xiaojun

PY - 2023/6

Y1 - 2023/6

N2 - Objective: Its eps gene cluster, the antioxidant activity and monosaccharide composition of exopolysaccharides, the expression levels of related genes at different fermentations were analyzed for clarifying the exopolysaccharide biosynthesis mechanism of Lactobacillus delbrueckii subsp. bulgaricus LDB-C1. Results: The comparison analysis of eps gene clusters indicated that the gene clusters present diversity and strain specificity. The crude exopolysaccharides from LDB-C1 exhibited a good antioxidant activity. Compared with glucose, fructose, galactose, and fructooligosaccharide, inulin significantly improved the exopolysaccharide biosynthesis. The structures of EPSs were significantly different under different carbohydrate fermentation conditions. Inulin obviously increased the expressions of most EPS biosynthesis related genes at fermentation 4 h. Conclusion: Inulin accelerated the beginning of the exopolysaccharide production in LDB-C1, and the enzymes promoted by inulin was beneficial for the accumulation of exopolysaccharide at the whole fermentation process.

AB - Objective: Its eps gene cluster, the antioxidant activity and monosaccharide composition of exopolysaccharides, the expression levels of related genes at different fermentations were analyzed for clarifying the exopolysaccharide biosynthesis mechanism of Lactobacillus delbrueckii subsp. bulgaricus LDB-C1. Results: The comparison analysis of eps gene clusters indicated that the gene clusters present diversity and strain specificity. The crude exopolysaccharides from LDB-C1 exhibited a good antioxidant activity. Compared with glucose, fructose, galactose, and fructooligosaccharide, inulin significantly improved the exopolysaccharide biosynthesis. The structures of EPSs were significantly different under different carbohydrate fermentation conditions. Inulin obviously increased the expressions of most EPS biosynthesis related genes at fermentation 4 h. Conclusion: Inulin accelerated the beginning of the exopolysaccharide production in LDB-C1, and the enzymes promoted by inulin was beneficial for the accumulation of exopolysaccharide at the whole fermentation process.

KW - Exopolysaccharide

KW - Inulin

KW - Lactobacillus delbrueckii subsp. bulgaricus

KW - Two component system

UR - https://www.scopus.com/pages/publications/85151639542

U2 - 10.1007/s10529-023-03365-z

DO - 10.1007/s10529-023-03365-z

M3 - 文章

C2 - 37010620

AN - SCOPUS:85151639542

SN - 0141-5492

VL - 45

SP - 639

EP - 654

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 5-6

ER -

Inulin increases the EPS biosynthesis of Lactobacillus delbrueckii ssp. bulgaricus LDB-C1

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Keywords

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