Introduction

Robotic micromanipulation is one of the most powerful cutting-edge technologies that has enabled significant explorations and discoveries in the micro- and nano- world, assisting researchers to directly manipulate small objects under microscopy. A number of novel and efficient methods in the field of micromanipulation have been developed such as micromanipulator equipped with end-effectors, as well as emerging untethered field-driven micromanipulation approaches, where the latter is seldom used in macro world due to their low driving capability. Leveraging robotic micromanipulation to explore micro world has significantly promoted a wide range of disciplines, particularly for almost all fields in modern biology. Among various kinds of biological objects, zebrafish and their larvae have been demonstrated one of the most popular model vertebrates due to their two main advantages: The whole-organism transparency that facilitates live imaging and the high production which is amenable to high-throughput operations. This chapter begins with a review of state-of-the-art results in robotic micromanipulation and then presents challenges in zebrafish larva-targeted micromanipulation.