Insight into the effects of sulfamethoxazole and norfloxacin on nitrogen transformation functional genes during swine manure composting

Zhiqiang Chen; Yiqi Wu; Qinxue Wen; Huanyu Bao; Qiqi Fu

doi:10.1016/j.biortech.2019.122463

Insight into the effects of sulfamethoxazole and norfloxacin on nitrogen transformation functional genes during swine manure composting

Zhiqiang Chen
, Yiqi Wu
, Qinxue Wen^*
, Huanyu Bao
, Qiqi Fu

^*Corresponding author for this work

School of Environment, Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The effects of sulfamethoxazole and norfloxacin on nitrogen functional genes were investigated in four composting treatments of swine manure: CK (no antibiotics), SMZ (spiked with 5 mg kg⁻¹ dry weight (DW) sulfamethoxazole), NOR (spiked with 5 mg kg⁻¹DW norfloxacin), and SN (spiked with 5 mg kg⁻¹DW sulfamethoxazole and 5 mg kg⁻¹DW norfloxacin). Antibiotics decreased relative abundance of bacterial amoA and nxrA, while increased nosZ/nirK. The decline in amoA/16S rRNA increased the total NH₃ emission in SMZ and NOR from 1027.05 to 1144.39 and 1278.22 mg kg⁻¹DW. The decrease of nxrA/16S rRNA enhanced the NO₂⁻-N content and N₂O emission in SMZ in the initial composting. Additionally, the increase in nosZ/nirK probably was the main reason for the lower N₂O emission in SN than other treatments in the cooling phase. The inhibition on nitrification process and increase in NH₃ emission resulted from antibiotics is worthy of attention in the future.

Original language	English
Article number	122463
Journal	Bioresource Technology
Volume	297
DOIs	https://doi.org/10.1016/j.biortech.2019.122463
State	Published - Feb 2020
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Antibiotics
Composting
Functional genes
Nitrogen transformation

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10.1016/j.biortech.2019.122463

Cite this

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title = "Insight into the effects of sulfamethoxazole and norfloxacin on nitrogen transformation functional genes during swine manure composting",

abstract = "The effects of sulfamethoxazole and norfloxacin on nitrogen functional genes were investigated in four composting treatments of swine manure: CK (no antibiotics), SMZ (spiked with 5 mg kg−1 dry weight (DW) sulfamethoxazole), NOR (spiked with 5 mg kg−1DW norfloxacin), and SN (spiked with 5 mg kg−1DW sulfamethoxazole and 5 mg kg−1DW norfloxacin). Antibiotics decreased relative abundance of bacterial amoA and nxrA, while increased nosZ/nirK. The decline in amoA/16S rRNA increased the total NH3 emission in SMZ and NOR from 1027.05 to 1144.39 and 1278.22 mg kg−1DW. The decrease of nxrA/16S rRNA enhanced the NO2−-N content and N2O emission in SMZ in the initial composting. Additionally, the increase in nosZ/nirK probably was the main reason for the lower N2O emission in SN than other treatments in the cooling phase. The inhibition on nitrification process and increase in NH3 emission resulted from antibiotics is worthy of attention in the future.",

keywords = "Antibiotics, Composting, Functional genes, Nitrogen transformation",

author = "Zhiqiang Chen and Yiqi Wu and Qinxue Wen and Huanyu Bao and Qiqi Fu",

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year = "2020",

month = feb,

doi = "10.1016/j.biortech.2019.122463",

language = "英语",

volume = "297",

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T1 - Insight into the effects of sulfamethoxazole and norfloxacin on nitrogen transformation functional genes during swine manure composting

AU - Chen, Zhiqiang

AU - Wu, Yiqi

AU - Wen, Qinxue

AU - Bao, Huanyu

AU - Fu, Qiqi

PY - 2020/2

Y1 - 2020/2

N2 - The effects of sulfamethoxazole and norfloxacin on nitrogen functional genes were investigated in four composting treatments of swine manure: CK (no antibiotics), SMZ (spiked with 5 mg kg−1 dry weight (DW) sulfamethoxazole), NOR (spiked with 5 mg kg−1DW norfloxacin), and SN (spiked with 5 mg kg−1DW sulfamethoxazole and 5 mg kg−1DW norfloxacin). Antibiotics decreased relative abundance of bacterial amoA and nxrA, while increased nosZ/nirK. The decline in amoA/16S rRNA increased the total NH3 emission in SMZ and NOR from 1027.05 to 1144.39 and 1278.22 mg kg−1DW. The decrease of nxrA/16S rRNA enhanced the NO2−-N content and N2O emission in SMZ in the initial composting. Additionally, the increase in nosZ/nirK probably was the main reason for the lower N2O emission in SN than other treatments in the cooling phase. The inhibition on nitrification process and increase in NH3 emission resulted from antibiotics is worthy of attention in the future.

AB - The effects of sulfamethoxazole and norfloxacin on nitrogen functional genes were investigated in four composting treatments of swine manure: CK (no antibiotics), SMZ (spiked with 5 mg kg−1 dry weight (DW) sulfamethoxazole), NOR (spiked with 5 mg kg−1DW norfloxacin), and SN (spiked with 5 mg kg−1DW sulfamethoxazole and 5 mg kg−1DW norfloxacin). Antibiotics decreased relative abundance of bacterial amoA and nxrA, while increased nosZ/nirK. The decline in amoA/16S rRNA increased the total NH3 emission in SMZ and NOR from 1027.05 to 1144.39 and 1278.22 mg kg−1DW. The decrease of nxrA/16S rRNA enhanced the NO2−-N content and N2O emission in SMZ in the initial composting. Additionally, the increase in nosZ/nirK probably was the main reason for the lower N2O emission in SN than other treatments in the cooling phase. The inhibition on nitrification process and increase in NH3 emission resulted from antibiotics is worthy of attention in the future.

KW - Antibiotics

KW - Composting

KW - Functional genes

KW - Nitrogen transformation

UR - https://www.scopus.com/pages/publications/85075899077

U2 - 10.1016/j.biortech.2019.122463

DO - 10.1016/j.biortech.2019.122463

M3 - 文章

C2 - 31786036

AN - SCOPUS:85075899077

SN - 0960-8524

VL - 297

JO - Bioresource Technology

JF - Bioresource Technology

M1 - 122463

ER -

Insight into the effects of sulfamethoxazole and norfloxacin on nitrogen transformation functional genes during swine manure composting

Abstract

UN SDGs

Keywords

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